Identification of basic fibroblast growth factor-like immunoreactivity in Panax ginseng extract: Investigation of its molecular properties

被引：7
|
作者：
Takei, Y [1 ]
Yamamoto, T [1 ]
Higashira, H [1 ]
Hayashi, K [1 ]
机构：
[1] GIFU PHARMACEUT UNIV, DEPT BIOL MOLEC, GIFU 502, JAPAN
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 04期
关键词：
basic fibroblast growth factor (bFGF); Panax ginseng; enzyme immunoassay (EIA); purification; mitogenic activity;
D O I：
10.1271/bbb.60.584
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Basic fibroblast growth factor (bFGF)-like immunoreactivity was detected in extracts of Panax ginseng root by using a sensitive two-site enzyme immunoassay specific for human bFGF (hbFGF). In an investigation of the molecular properties of this bFGF-like molecule (bFGF-LI), the bFGF-LI and hbFGF were found to be equivalent with respect to antigenicity, molecular weight, isoelectric point, affinity for binding to heparin, and mitogenic activity toward BALB/c3T3 fibroblasts. The identification of this bFGF-LI molecule in Panax ginseng root helps to explain various activities of the traditional Chinese medicine ginseng.
引用
收藏
页码：584 / 588
页数：5
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