Anti-inflammatory effects of aucubin in cellular and animal models of rheumatoid arthritis

被引:9
作者
Zhang Yan [1 ]
Tang Li-Dong [1 ]
Wang Jian-Ying [2 ]
Wang Hao [3 ]
Chen Xiao-Yun [4 ]
Zhang Lei [2 ]
Yuan Ying [1 ]
机构
[1] Shanghai Univ Tradit Chinese Med, Sch Tradit Chinese Mat Med, Shanghai 201203, Peoples R China
[2] Shanghai Univ Tradit Chinese Med, Shanghai Innovat Ctr TCM Hlth Serv, Shanghai 201203, Peoples R China
[3] Shanghai Univ Tradit Chinese Med, Shanghai Key Lab Formulated Chinese Med, Shanghai 201203, Peoples R China
[4] Shanghai Univ, Shanghai Longhua Hosp, TCM Rheumatoid Dept, Shanghai 200232, Peoples R China
关键词
Collagen-induced arthritis; Synovial inflammation; Bone erosion; NF-?B signaling pathway; Aucubin; NF-KAPPA-B; FIBROBLAST-LIKE SYNOVIOCYTES; IN-VITRO; INFLAMMATION; DIFFERENTIATION; EXPRESSION; APOPTOSIS; CELLS;
D O I
10.1016/S1875-5364(22)60182-1
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease. It is known that aucubin (AU) exerts anti-inflammatory activity, but its effects and mechanisms in RA are unclear. This study investigated the anti-inflammatory effects and mechanisms of AU in vivo and in vitro. Human fibroblast-like synoviocyte cells from patients with RA (HFLS-RA), RAW264.7 cells, and MC3T3-E1 cells were used to evaluate the effects of AU on migration, invasion, apoptosis, osteoclast differentiation and production. Immunofluorescence was used to observe nuclear translocation of nuclear factor (NF)-Kappa B, the double luciferase reporter gene method was used to observe NF-Kappa B-p65 activity in AU-treated MC3T3-E1 cells. RT-qPCR was used to measure expression of bone metabolism and inflammation-related genes, and western blot was used to measure bone metabolism and NF-Kappa B protein expression levels. Collagen-induced arthritis (CIA) rat model was used for pharmacodynamics study. Arthritis indexes were measured in the ankle and knee, histological staining and Micro-computed tomography were performed on the ankle joints. Also, inflammatory factor gene expression and the levels of NF-Kappa B-related proteins were detected as in vitro. AU effectively inhibited HFLS-RA cell migration and invasion, promoted apoptosis, and inhibited RAW264.7 cell differentiation into osteoclasts, as well as inhibited NF-Kappa B-p65 activity in MC3T3-E1 cells. Notably, AU significantly reduced the gene expression levels of three cell-related inflammatory factors and bone metabolism factors, effectively inhibited the expression of p-I Kappa Kappa alpha beta, p-I Kappa B alpha, and p-p65 proteins. In vivo, AU relieved joint inflammation, reduced related inflammatory factors, and inhibited NF-Kappa B signaling. It could be used to treat RA-related synovial inflammation and bone destruction through the NF-Kappa B pathway. [CLC Number] R965 [Document code] A [Article ID] 2095-6975(2022)06-0458-15
引用
收藏
页码:458 / 472
页数:15
相关论文
共 42 条
[1]   Fibroblast-like synoviocytes: key effector cells in rheumatoid arthritis [J].
Bartok, Beatrix ;
Firestein, Gary S. .
IMMUNOLOGICAL REVIEWS, 2010, 233 :233-255
[2]   Taraxasterol suppresses inflammation in IL-1β-induced rheumatoid arthritis fibroblast-like synoviocytes and rheumatoid arthritis progression in mice [J].
Chen, Jianfeng ;
Wu, Weibo ;
Zhang, Miaomiao ;
Chen, Caiming .
INTERNATIONAL IMMUNOPHARMACOLOGY, 2019, 70 :274-283
[3]   Caffeine enhances osteoclast differentiation and maturation through p38 MAP kinase/Mitf and DC-STAMP/CtsK and TRAP pathway [J].
Choi, Jiwon ;
Choi, So Yoen ;
Lee, Sun Young ;
Lee, Jae Yoon ;
Kim, Hong Sung ;
Lee, Soo Young ;
Lee, Na Kyung .
CELLULAR SIGNALLING, 2013, 25 (05) :1222-1227
[4]   Experimental animal models for rheumatoid arthritis [J].
Choudhary, Narayan ;
Bhatt, Lokesh K. ;
Prabhavalkar, Kedar S. .
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY, 2018, 40 (03) :193-200
[5]  
Cope A.P., 2019, CLIN IMMUNOL, P705, DOI [10.1016/B978-0-7020-6896-6.00052-1, DOI 10.1016/B978-0-7020-6896-6.00052-1]
[6]  
Czekanska EM, 2012, EUR CELLS MATER, V24, P1
[7]  
Navarro-Hernández RE, 2009, DIS MARKERS, V26, P119, DOI [10.1155/2009/510104, 10.3233/DMA-2009-0621]
[8]   The pathogenic role of angiogenesis in rheumatoid arthritis [J].
Elshabrawy, Hatem A. ;
Chen, Zhenlong ;
Volin, Michael V. ;
Ravella, Shalini ;
Virupannavar, Shanti ;
Shahrara, Shiva .
ANGIOGENESIS, 2015, 18 (04) :433-448
[9]   Serum immunoglobulin free light chain levels in systemic autoimmune rheumatic diseases [J].
Gulli, F. ;
Napodano, C. ;
Marino, M. ;
Ciasca, G. ;
Pocino, K. ;
Basile, V. ;
Visentini, M. ;
Stefanile, A. ;
Todi, L. ;
De Spirito, M. ;
Rapaccini, G. L. ;
Basile, U. .
CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2020, 199 (02) :163-171
[10]  
[韩曼飞 Han Manfei], 2017, [中草药, Chinese Traditional and Herbal Drugs], V48, P4105