Ultra-sensitive method based on time-resolved fluorescence immunoassay for detection of sulfamethazine in raw milk

被引：17

作者：

Hu, Song ^{[1
]}

Li, Danhong ^{[2
]}

Huang, Zhen ^{[1
]}

Xing, Keyu ^{[1
]}

Chen, Yuan ^{[1
]}

Peng, Juan ^{[2
]}

Lai, Weihua ^{[1
]}

机构：

[1] Nanchang Univ, State Key Lab Food Sci & Technol, 235 Nanjing East Rd, Nanchang 330047, Jiangxi, Peoples R China

[2] Nanchang Univ, Sch Food Sci, 235 Nanjing East Rd, Nanchang 330047, Jiangxi, Peoples R China

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2018年 / 29卷 / 01期

基金：

美国国家科学基金会;

关键词：

Lateral flow assay; Eu (III)-doped polystyrene nanoparticle; sulfamethazine; raw milk; ESCHERICHIA-COLI O157H7; IMMUNOCHROMATOGRAPHIC ASSAY; LIQUID-CHROMATOGRAPHY; SULFONAMIDE RESIDUES; MONOCLONAL-ANTIBODY; FLUOROIMMUNOASSAY; FLORFENICOL; EXTRACTION; TISSUES; HAPTEN;

D O I：

10.1080/09540105.2018.1520816

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A novel lateral flow assay (LFA) was developed by introducing Eu (III)-doped polystyrene nanoparticles (EuNPs) for rapid and ultra-sensitive detection of sulfamethazine (SM2) in raw milk. The limit of detection and linear range of the proposed method were 0.0045 and 0.05-10ng/mL, respectively. The recovery of LFA for the detection of SM2 in raw milk was 96.1-108.2%. The proposed LFA provides a rapid and convenient strategy for fast and ultra-sensitive screening of SM2 in raw milk. EuNP-LFA may be a remarkable method for the detection of other targets at low concentrations to ensure food safety. [GRAPHICS]

引用

页码：1137 / 1149

页数：13

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