Identification of a functional SNP in the 3-UTR of caprine MTHFR gene that is associated with milk protein levels

被引：10

作者：

An, Xiaopeng ^{[1
]}

Song, Yuxuan ^{[1
]}

Hou, Jinxing ^{[1
]}

Wang, Shan ^{[1
]}

Gao, Kexin ^{[1
]}

Cao, Binyun ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Anim Sci & Technol, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China

来源：

ANIMAL GENETICS | 2016年 / 47卷 / 04期

基金：

中国博士后科学基金;

关键词：

breeding; DNA sequencing; gene mutation; miRNAs; EXPRESSION; SITE;

D O I：

10.1111/age.12425

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Xinong Saanen (n=305) and Guanzhong (n=317) dairy goats were used to detect SNPs in the caprine MTHFR 3-UTR by DNA sequencing. One novel SNP (c.*2494G>A) was identified in the said region. Individuals with the AA genotype had greater milk protein levels than did those with the GG genotype at the c.*2494G>A locus in both dairy goat breeds (P<0.05). Functional assays indicated that the MTHFR:c.2494G>A substitution could increase the binding activity of bta-miR-370 with the MTHFR 3-UTR. In addition, we observed a significant increase in the MTHFR protein level of AA carriers relative to that of GG carriers. These altered levels of MTHFR protein may account for the association of the SNP with milk protein level.

引用

页码：499 / 503

页数：5

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