Screening of the miRNAs related to breast cancer and identification of its target genes

被引：0

作者：

Sun, Xianfu ^{[1
]}

Luo, Suxia ^{[2
]}

He, Yaning ^{[1
]}

Shao, Yingbo ^{[1
]}

Liu, Chaojun ^{[1
]}

Chen, Qi ^{[1
]}

Cui, Shude ^{[1
]}

Liu, Hui ^{[1
]}

机构：

[1] Zhengzhou Univ, Affiliated Canc Hosp, Dept Breast, Henan Canc Hosp, Zhengzhou 450008, Peoples R China

[2] Zhengzhou Univ, Affiliated Canc Hosp, Dept Oncol, Henan Canc Hosp, Zhengzhou 450008, Peoples R China

来源：

EUROPEAN JOURNAL OF GYNAECOLOGICAL ONCOLOGY | 2014年 / 35卷 / 06期

关键词：

Breast cancer; miRNAs; Microarray; miR-21; miR-200c; Target gene; EXPRESSION; MICRORNAS; METASTASIS; CELLS; RNAS;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Objective: To predict and verify the target genes of the miRNAs related to breast cancer beginning from the miRNA expression profile of human breast cancer. Materials and Methods: The total RNA was extracted from cancer tissues and the corresponding paracancerous tissues of eight breast cancer patients, and then miRNAs were separated. Human breast cancer cell line MDA-MB-231 and the normal mammary epithelial cell line BBL-100 were cultured, and the total RNA was extracted, respectively, with separation of miRNAs. The gene chip technology was used to analyze and detect the miRNAs differentially expressed in tissues and cancer cells. The miRNA expression profile of human breast cancer was obtained through chip scanning and data analysis. Results: Through dual-luciferase method, it was verified that PDCD4 and PDCD10 were real target genes of miR-21 and miR-200c, respectively. Conclusion: tniR-21 and miR-200c are related miRNAs to breast cancer, and they are associated with the occurrence and development of breast cancer.

引用

页码：696 / 700

页数：5

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