Gelatin filter capture-based high-throughput sequencing analysis of microbial diversity in haze particulate matter

被引：0
|
作者：
Sun, Meiqing [1 ,2 ]
Ding, Zhanlin [1 ]
Wang, Hong [1 ]
Yu, Guangping [1 ]
Feng, Zhe [1 ]
Li, Bingzhi [2 ]
Li, Penghui [3 ]
机构：
[1] Wuqing Dist Ctr Dis Control & Prevent, Tianjin 301700, Peoples R China
[2] Tianjin Univ, Sch Chem Engn & Technol, Key Lab Syst Bioengn, Minist Educ, Tianjin 300072, Peoples R China
[3] Tianjin Univ Technol, Sch Environm Sci & Safety Engn, Tianjin 300384, Peoples R China
来源：
JOURNAL OF BIOMEDICAL RESEARCH | 2019年 / 33卷 / 06期
关键词：
airborne particulate matter; gelatin filter; pathogen; high-throughput sequencing; RESISTANT STAPHYLOCOCCUS-AUREUS; HEALTH-RISK ASSESSMENT; AIR-POLLUTION; HEAVY-METALS; PM2.5; EXPOSURE; HOSPITALS; BIOAEROSOL; BACTERIAL; AEROSOLS;
D O I：
10.7555/JBR.33.20180121
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Airborne particulate matter (PM), especially PM2.5, can be easily adsorbed by human respiratory system. Their roles in carrying pathogens for spreading epidemic diseases has attracted great concern. Herein, we developed a novel gelatin filter-based and culture-independent method for investigation of the microbial diversity in PM samples during a haze episode in Tianjin, China. This method involves particle capture by gelatin filters, filter dissolution for DNA extraction, and high-throughput sequencing for analysis of the microbial diversity. A total of 584 operational taxonomic units (OTUs) of bacteria and 370 OTUs of fungi at the genus level were identified during hazy days. The results showed that both bacterial and fungal diversities could be evaluated by this method. This study provides a convenient strategy for investigation of microbial biodiversity in haze, facilitating accurate evaluation of airborne epidemic diseases.
引用
收藏
页码：414 / 421
页数：8
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