Immunogenicity of virus-like Semliki Forest virus replicon particles expressing Indian HIV-1C gag, env and polRT genes

被引：11

作者：

Ajbani, Seema P. ^{[1
,2
]}

Velhal, Shilpa M. ^{[1
]}

Kadam, Ravindra B. ^{[1
]}

Patel, Vainav V. ^{[1
]}

Lundstrom, Kenneth ^{[3
]}

Bandivdekar, Atmaram H. ^{[1
]}

机构：

[1] Natl Inst Res Reprod Hlth, Dept Biochem & Virol, JM St, Mumbai 400012, Maharashtra, India

[2] Univ Mumbai, Dept Zool, Smt CHM Coll, Ulhasnagar 421003, Maharashtra, India

[3] PanTherapeutics, Lutry, Switzerland

来源：

IMMUNOLOGY LETTERS | 2017年 / 190卷

关键词：

Semliki Forest virus; HIV-1 subtype C (HIV-1C); Virus-like Replicon Particle; T cell response; Humoral response; T-CELL RESPONSES; HUMORAL IMMUNE-RESPONSES; LOUPING-ILL VIRUS; SUBTYPE-C VACCINE; VIRAL LOAD; CYNOMOLGUS MONKEYS; DENDRITIC CELLS; EFFICACY TRIAL; BOOST REGIMEN; DOUBLE-BLIND;

D O I：

10.1016/j.imlet.2017.08.019

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Development of a vaccine targeting human immunodeficiency virus-1 subtype C (HIV-1C) is an important public health priority in regions with a high prevalence of the Glade C virus. The present study demonstrates the immunogenicity of recombinant Semliki Forest virus (SFV)-based virus-like replicon particles (VRPs) expressing Indian HIV-1C env/gag/polRT genes. Immunization of mice with recombinant VRPs in a homologous prime-boost protocol, either individually or in combination, elicited significant antigen-specific IFN-gamma T cell responses as detected by the ELISPOT assay. Additionally, Gag-specific TNF-alpha secreting CD8(+) and CD4(+) T cells and Env-specific IL-2 secreting T cells were also elicited by mice immunized with Gag and Env constructs, respectively, as estimated by intracellular cytokine staining assay. Moreover, an HIV Pol-specific TNF-alpha response was elicited in mice immunized with a combination of the three VRP constructs. Furthermore, HIV-1C Gag and Env-specific binding antibodies were elicited as verified by gp120 ELISA and p24 Gag ELISA, respectively. The immunogenicity of VRPs was found to be higher as compared to that of RNA replicons and VRPs may therefore be promising preventive and therapeutic candidate vaccines for the control and management of HIV/AIDS.

引用

页码：221 / 232

页数：12

共 54 条

[11] Efficacy assessment of a cell-mediated immunity HIV-1 vaccine (the Step Study): a double-blind, randomised, placebo-controlled, test-of-concept trial [J].

Buchbinder, Susan P. ;

Mehrotra, Devon V. ;

Duerr, Ann ;

Fitzgerald, Daniel W. ;

Mogg, Robin ;

Li, David ;

Gilbert, Peter B. ;

Lama, Javier R. ;

Marmor, Michael ;

del Rio, Carlos ;

McElrath, M. Juliana ;

Casimiro, Danilo R. ;

Gottesdiener, Keith M. ;

Chodakewitz, Jeffrey A. ;

Corey, Lawrence ;

Robertson, Michael N. .

LANCET, 2008, 372 (9653) :1881-1893

[12] Generation and immunogenicity of novel HIV/AIDS vaccine candidates targeting HIV-1 Env/Gag-Pol-Nef antigens of clade C [J].

Elena Gomez, Carmen ;

Luis Najera, Jose ;

Jimenez, Victoria ;

Bieler, Kurt ;

Wild, Jens ;

Kostic, Linda ;

Heidari, Shirin ;

Chen, Margaret ;

Frachette, Marie-Joelle ;

Pantaleo, Giuseppe ;

Wolf, Hans ;

Liljestrom, Peter ;

Wagner, Ralf ;

Esteban, Mariano .

VACCINE, 2007, 25 (11) :1969-1992

[13] Recombinant Semliki Forest virus particles encoding the prME or NS1 proteins of louping ill virus protect mice from lethal challenge [J].

Fleeton, MN ;

Sheahan, BJ ;

Gould, EA ;

Atkins, GJ ;

Liljeström, P .

JOURNAL OF GENERAL VIROLOGY, 1999, 80 :1189-1198

[14] Self-replicative RNA vaccines elicit protection against influenza A virus, respiratory syncytial virus, and a tickborne encephalitis virus [J].

Fleeton, MN ;

Chen, M ;

Berglund, P ;

Rhodes, G ;

Parker, SE ;

Murphy, M ;

Atkins, GJ ;

Liljeström, P .

JOURNAL OF INFECTIOUS DISEASES, 2001, 183 (09) :1395-1398

[15] Modified Vaccinia Virus Ankara-Based Vaccine Vectors Induce Apoptosis in Dendritic Cells Draining from the Skin via both the Extrinsic and Intrinsic Caspase Pathways, Preventing Efficient Antigen Presentation [J].

Guzman, E. ;

Cubillos-Zapata, C. ;

Cottingham, M. G. ;

Gilbert, S. C. ;

Prentice, H. ;

Charleston, B. ;

Hope, J. C. .

JOURNAL OF VIROLOGY, 2012, 86 (10) :5452-5466

[16] Construction and immunogenicity in a prime-boost regimen of a Semliki Forest virus-vectored experimental HIV clade A vaccine [J].

Hanke, T ;

Barnfield, C ;

Wee, EGT ;

Ågren, L ;

Samuel, RV ;

Larke, N ;

Liljeström, P .

JOURNAL OF GENERAL VIROLOGY, 2003, 84 :361-368

[17]

Harrer T, 1996, J IMMUNOL, V156, P2616

[18] Immunization of rhesus macaques with a DNA prime/modified vaccinia virus Ankara boost regimen induces broad simian immunodeficiency virus (SIV)-specific T-cell responses and reduces initial viral replication but does not prevent disease progression following challenge with pathogenic SIVmac239 [J].

Horton, H ;

Vogel, TU ;

Carter, DK ;

Vielhuber, K ;

Fuller, DH ;

Shipley, T ;

Fuller, JT ;

Kunstman, KJ ;

Sutter, G ;

Montefiori, DC ;

Erfle, V ;

Desrosiers, RC ;

Wilson, N ;

Picker, LJ ;

Wolinsky, SM ;

Wang, CX ;

Allison, DB ;

Watkins, DI .

JOURNAL OF VIROLOGY, 2002, 76 (14) :7187-7202

[19] The Thai Phase III HIV Type 1 Vaccine Trial (RV144) Regimen Induces Antibodies That Target Conserved Regions Within the V2 Loop of gp120 [J].

Karasavvas, Nicos ;

Billings, Erik ;

Rao, Mangala ;

Williams, Constance ;

Zolla-Pazner, Susan ;

Bailer, Robert T. ;

Koup, Richard A. ;

Madnote, Sirinan ;

Arworn, Duangnapa ;

Shen, Xiaoying ;

Tomaras, Georgia D. ;

Currier, Jeffrey R. ;

Jiang, Mike ;

Magaret, Craig ;

Andrews, Charla ;

Gottardo, Raphael ;

Gilbert, Peter ;

Cardozo, Timothy J. ;

Rerks-Ngarm, Supachai ;

Nitayaphan, Sorachai ;

Pitisuttithum, Punnee ;

Kaewkungwal, Jaranit ;

Paris, Robert ;

Greene, Kelli ;

Gao, Hongmei ;

Gurunathan, Sanjay ;

Tartaglia, Jim ;

Sinangil, Faruk ;

Korber, Bette T. ;

Montefiori, David C. ;

Mascola, John R. ;

Robb, Merlin L. ;

Haynes, Barton F. ;

Ngauy, Viseth ;

Michael, Nelson L. ;

Kim, Jerome H. ;

de Souza, Mark S. .

AIDS RESEARCH AND HUMAN RETROVIRUSES, 2012, 28 (11) :1444-1457

[20] Vaccine protection from CD4+ T-cell loss caused by simian immunodeficiency virus (SIV) mac251 is afforded by sequential immunization with three unrelated vaccine vectors encoding multiple SIV antigens [J].

Koopman, G ;

Mortier, D ;

Hofman, S ;

Niphuis, H ;

Fagrouch, Z ;

Norley, S ;

Sutter, G ;

Liljeström, P ;

Heeney, JL .

JOURNAL OF GENERAL VIROLOGY, 2004, 85 :2915-2924

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