Purification and biochemical characterization of an endoxylanase from Aspergillus versicolor

被引：18

作者：

Carmona, EC

Brochetto-Braga, MR

Pizzirani-Kleiner, AA

Jorge, JA ^{[1
]}

机构：

[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Pret, Dept Biol, BR-14040901 Ribeirao Preto, SP, Brazil

[2] UNESP, Inst Biociencia, Dept Bioquim & Microbiol, BR-13506900 Sao Paulo, Brazil

来源：

FEMS MICROBIOLOGY LETTERS | 1998年 / 166卷 / 02期

关键词：

xylanase; endoxylanase; Aspergillus versicolor;

D O I：

10.1111/j.1574-6968.1998.tb13906.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An endoxylanase (beta-1,4-xylan xylanohydrolase, EC 3.2.1.8) was purified from the culture filtrate of a strain of Aspergillus versicolor grown on oat wheat. The enzyme was purified to homogeneity by chromatography on DEAE-cellulose and Sephadex G-75. The purified enzyme was a monomer of molecular mass estimated to be 19 kDa by SDS-PAGE and gel filtration. The enzyme was glycoprotein with 71% carbohydrate content and exhibited a pI of 5.4. The purified xylanase was specific for xylan hydrolysis. The enzyme had a K-m of 6.5 mg ml(-1) and a V-max of 1440 U (mg protein)(-1). (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：311 / 315

页数：5

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