Recent advances in the discovery, characterization, and engineering of poly (ethylene terephthalate) (PET) hydrolases

被引：56
作者：
Gao, Rui [1 ]
Pan, Haojie [1 ]
Lian, Jiazhang [1 ,2 ]
机构：
[1] Zhejiang Univ, Coll Chem & Biol Engn, Key Lab Biomass Chem Engn, Minist Educ, Hangzhou 310027, Peoples R China
[2] Zhejiang Univ, Hangzhou Global Sci & Technol Innovat Ctr, Hangzhou 310027, Peoples R China
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 2021年 / 150卷
关键词：
Poly(ethylene terephthalate); PET; PET hydrolase; PET biodegradation; Protein engineering; Recombinant protein expression system; CUTINASE-CATALYZED HYDROLYSIS; ENZYMATIC SURFACE HYDROLYSIS; POLYETHYLENE TEREPHTHALATE; CRYSTAL-STRUCTURE; ESCHERICHIA-COLI; POLYESTER HYDROLASES; KINETIC-PROPERTIES; BRANCH COMPOST; FOLD ENZYMES; LIPASE-B;
D O I：
10.1016/j.enzmictec.2021.109868
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Poly(ethylene terephthalate) (PET) is a class of polyester plastic composed of terephthalic acid (TPA) and ethylene glycol (EG). The accumulation of large amount of PET waste has resulted in severe environmental and health problems. Microbial polyester hydrolases with the ability to degrade PET provide an economy- and environment-friendly approach for the treatment of PET waste. In recent years, many PET hydrolases have been discovered and characterized from various microorganisms and engineered for better performance under practical application conditions. Here, recent progress in the discovery, characterization, and enzymatic mechanism elucidation of PET hydrolases is firstly reviewed. Then, structure-guided protein engineering of PET hydrolases with increased enzymatic activities, expanded substrate specificity, as well as improved protein stability is summarized. In addition, strategies for efficient expression of recombinant PET hydrolases, including secretory expression and cell-surface display, are briefly introduced. This review is concluded with future perspectives in biodegradation and subsequent biotransformation of PET wastes to produce value-added compounds.
引用
收藏
页数：11
相关论文
共 125 条
[1]   Enzymatic Surface Hydrolysis of PET: Effect of Structural Diversity on Kinetic Properties of Cutinases from Thermobifida [J].
Acero, Enrique Herrero ;
Ribitsch, Doris ;
Steinkellner, Georg ;
Gruber, Karl ;
Greimel, Katrin ;
Eiteljoerg, Inge ;
Trotscha, Eva ;
Wei, Ren ;
Zimmermann, Wolfgang ;
Zinn, Manfred ;
Cavaco-Paulo, Artur ;
Freddi, Giuliano ;
Schwab, Helmut ;
Guebitz, Georg .
MACROMOLECULES, 2011, 44 (12) :4632-4640
[2]   Atmospheric transport and deposition of microplastics in a remote mountain catchment [J].
Allen, Steve ;
Allen, Deonie ;
Phoenix, Vernon R. ;
Le Roux, Gael ;
Jimenez, Pilar Durantez ;
Simonneau, Anaelle ;
Binet, Stephane ;
Galop, Didier .
NATURE GEOSCIENCE, 2019, 12 (05) :339-+
[3]   Glass transition and structural relaxation in semi-crystalline poly(ethylene terephthalate):: a DSC study [J].
Alves, NM ;
Mano, JF ;
Balaguer, E ;
Dueñas, JMM ;
Ribelles, JLG .
POLYMER, 2002, 43 (15) :4111-4122
[4]   Applications and societal benefits of plastics [J].
Andrady, Anthony L. ;
Neal, Mike A. .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2009, 364 (1526) :1977-1984
[5]   Tailoring cutinase activity towards polyethylene terephthalate and polyamide 6,6 fibers [J].
Araujo, Rita ;
Silva, Carla ;
O'Neill, Alexandre ;
Micaelo, Nuno ;
Guebitz, Georg ;
Soares, Claudio M. ;
Casal, Margarida ;
Cavaco-Paulo, Artur .
JOURNAL OF BIOTECHNOLOGY, 2007, 128 (04) :849-857
[6]   Bacterial lipolytic enzymes: classification and properties [J].
Arpigny, JL ;
Jaeger, KE .
BIOCHEMICAL JOURNAL, 1999, 343 :177-183
[7]   Characterization and engineering of a plastic-degrading aromatic polyesterase [J].
Austin, Harry P. ;
Allen, Mark D. ;
Donohoe, Bryon S. ;
Rorrer, Nicholas A. ;
Kearns, Fiona L. ;
Silveira, Rodrigo L. ;
Pollard, Benjamin C. ;
Dominick, Graham ;
Duman, Ramona ;
El Omari, Kamel ;
Mykhaylyk, Vitaliy ;
Wagner, Armin ;
Michener, William E. ;
Amore, Antonella ;
Skaf, Munir S. ;
Crowley, Michael F. ;
Thorne, Alan W. ;
Johnson, Christopher W. ;
Woodcock, H. Lee ;
McGeehan, John E. ;
Beckham, Gregg T. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2018, 115 (19) :E4350-E4357
[8]   Identification and comparison of cutinases for synthetic polyester degradation [J].
Baker, Peter James ;
Poultney, Christopher ;
Liu, Zhiqiang ;
Gross, Richard ;
Montclare, Jin Kim .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2012, 93 (01) :229-240
[9]   Enzymatic hydrolysis of polyethylene terephthalate films in an ultrafiltration membrane reactor [J].
Barth, Markus ;
Wei, Ren ;
Oeser, Thorsten ;
Then, Johannes ;
Schmidt, Juliane ;
Wohlgemuth, Franziska ;
Zimmermann, Wolfgang .
JOURNAL OF MEMBRANE SCIENCE, 2015, 494 :182-187
[10]   Effect of hydrolysis products on the enzymatic degradation of polyethylene terephthalate nanoparticles by a polyester hydrolase from Thermobifida fusca [J].
Barth, Markus ;
Oeser, Thorsten ;
Wei, Ren ;
Then, Johannes ;
Schmidt, Juliane ;
Zimmermann, Wolfgang .
BIOCHEMICAL ENGINEERING JOURNAL, 2015, 93 :222-228
12345678910