Ultrasensitive Electrochemical Detection of Prostate-Specific Antigen (PSA) Based on Graphene-Silver Nanocomposite

被引：4

作者：

Yu, Lei ^{[1
]}

Fang, Zhen ^{[2
]}

Li, Wenxian ^{[3
]}

Li, Shenqian ^{[1
]}

Wang, Peitao ^{[1
]}

Wang, Hongqiang ^{[1
]}

机构：

[1] Qingdao Univ, Dept Androl, Affiliated Hosp, 16 Jiangsu Rd, Qingdao, Peoples R China

[2] Qingdao Haici Med Grp, Dept Ophthalmol, Qingdao 266000, Shandong, Peoples R China

[3] Qingdao Univ, Dept Urol, Affiliated Hosp, 16 Jiangsu Rd, Qingdao, Shandong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE | 2017年 / 12卷 / 09期

关键词：

Prostate-specific antigen; Electrochemical sensor; Graphene; Silver; Children asthmatic Layer-by-layer; IMMUNOSENSOR; IMMUNOASSAY; BIOSENSOR; SENSOR; STRATEGY; ASSAY;

D O I：

10.20964/2017.09.62

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

In this work, the electrostatic layer-by-layer (LbL) assembly of bovine serum albumin (BSA) functionalized graphene nanosheets and silver nanoparticle (AgNP), along with the thermal annealing at 340 degrees C in air were performed to prepare a thin and uniform film of AgNP embedded porous graphene (AgEPG). To determine prostate specific antigen (PSA) in human serum, we proposed a new electrochemical immunosensor based on a protein tracer, namely the functionalized AgEPG. Our proposed immunosensor was feasible to detect PSA (1-35 ng/mL) under optimal electrochemical and immunoreactions determination conditions, and the limit of detection (LOD) was calculated as 0.44 ng/mL.

引用

页码：8188 / 8197

页数：10