Occurrence and Antimicrobial Resistance Profiles of Campylobacter jejuni, Campylobacter coli, and Campylobacter upsaliensis in Beef Cattle on Cow-Calf Operations in South Africa

被引：20

作者：

Karama, Musafiri ^{[1
]}

Kambuyi, Katembue ^{[1
]}

Cenci-Goga, Beniamino T. ^{[2
]}

Malahlela, Mogaugedi ^{[1
]}

Jonker, Annelize ^{[3
]}

He, Cheng ^{[4
,5
]}

Ombui, Jackson ^{[6
]}

Tshuma, Takula ^{[7
]}

Etter, Eric ^{[7
,8
]}

Kalake, Alan ^{[9
]}

机构：

[1] Univ Pretoria, Fac Vet Sci, Dept Paraclin Sci, Vet Publ Hlth Sect, P Bag X04, ZA-0110 Onderstepoort, South Africa

[2] Univ Perugia, Lab Ispez Alimenti Origine Anim, Dipartimento Med Vet, Perugia, Italy

[3] Univ Pretoria, Fac Vet Sci, Dept Trop Dis, Onderstepoort, South Africa

[4] Foshan Univ, Coll Life Sci & Engn, Foshan, Peoples R China

[5] China Agr Univ, Coll Vet Med, Key Lab Anim Epidemiol & Zoonosis, Minist Agr, Beijing, Peoples R China

[6] Univ Nairobi, Coll Agr & Vet Sci, Dept Publ Hlth Pharmacol & Toxicol, Nairobi, Kenya

[7] Univ Pretoria, Fac Vet Sci, Dept Prod Anim Studies, Onderstepoort, South Africa

[8] Univ Montpellier, Ctr Cooperat Int Rech Agron Dev, INRA, UMR ASTRE Baillarguet Int Campus, Montpellier, France

[9] Gauteng Dept Agr & Rural Dev, Johannesburg, South Africa

来源：

FOODBORNE PATHOGENS AND DISEASE | 2020年 / 17卷 / 07期

基金：

新加坡国家研究基金会;

关键词：

Campylobacter spp; beef cattle; antimicrobial resistance; South Africa; FEEDLOT CATTLE; PREVALENCE; BOVINE; SPP; HUMANS; RISK; MEAT;

D O I：

10.1089/fpd.2019.2703

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

This study investigated occurrence and antimicrobial resistance profiles of Campylobacter spp. isolates in beef cattle on five cow-calf operations in South Africa. A total of 537 fecal samples from adult beef cattle (n = 435) and rectal swabs from calves (n = 102) were screened for Campylobacter jejuni, Campylobacter coli, and Campylobacter upsaliensis by culture and polymerase chain reaction. Furthermore, 86 Campylobacter spp. isolates including 46 C. jejuni, 24 C. coli, and 16 C. upsaliensis were tested for antimicrobial resistance against a panel of 9 antimicrobials. Overall, Campylobacter spp. was detected in 29.7% of cattle. Among the 158 Campylobacter spp.-positive cattle, 61.8% carried C. jejuni, 25% carried C. coli, and 10% carried C. upsaliensis. Five animals (3.1%) had mixed infections: three cows carried C. jejuni and C. coli concurrently, one cow had both C. jejuni and C. upsaliensis, and one cow harbored C. coli and C. upsaliensis. Antimicrobial resistance profiling among 86 Campylobacter spp. isolates revealed that 52.3% of the isolates were resistant to one or more antimicrobials. Antimicrobial resistance was observed in 46.7% of C. jejuni isolates, 35.6% of C. coli, and 17.8% of C. upsaliensis. Thirty-six percent of isolates were resistant to clindamycin, 19.7% to nalidixic acid, 18.6% to tetracycline, and 17.4% to erythromycin. Lower resistance rates were recorded for azithromycin (8.1%), florfenicol (3.4%), gentamicin (4.8%), and telithromycin and ciprofloxacin (5.8%). Multidrug resistance (MDR) was observed in 32.5% of isolates. Significantly higher levels of MDR were detected among C. jejuni (36.9%) and C. coli (33.3%) isolates in comparison to C. upsaliensis (18.7%). Two main multiresistance patterns were detected: nalidixic acid/clindamycin (17.8%) and tetracycline/clindamycin (14.2%). To the best of our knowledge, this is the first study which has shown that beef cattle on cow-calf operations in South Africa constitute an important reservoir and a potential source of clinically relevant and antimicrobial resistant Campylobacter spp. strains.

引用

页码：440 / 446

页数：7

共 44 条

[1] Campylobacteriosis in dogs and cats: a review [J].