HPLC and solubility study of the interaction between pindolol and cyclodextrins

被引：27

作者：

Gazpio, C ^{[1
]}

Sánchez, M ^{[1
]}

García-Zubiri, IX ^{[1
]}

Vélaz, I ^{[1
]}

Martínez-Ohárriz, C ^{[1
]}

Martín, C ^{[1
]}

Zornoza, A ^{[1
]}

机构：

[1] Univ Navarra, Fac Ciencias, Dept Quim & Edafol, E-31080 Pamplona, Spain

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2005年 / 37卷 / 03期

关键词：

pindolol; cyclodextrin; HPLC; complex formation; solubility isotherm;

D O I：

10.1016/j.jpba.2004.11.008

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The complexation with beta-cyclodextrin (beta-CD) has been investigated using reversed-phase liquid chromatography. The compounds tested have been pindolol and, for comparison purposes, indole and 4-methoxyindole. The retention behaviour has been analysed on a Kromasil 100 C18 column and the mobile phase used was methanol-pH 6 phosphate buffer (15/85 v/v) in which beta-CD was incorporated as a mobile phase additive. The decrease in the retention times with increasing concentrations of beta-CD enables the determination of the apparent stability constants of the complexes. In addition, the low solubility of pindolol, a weak base, in pH 12 aqueous solution has been improved by complexation with different cyclodextrins. The solubility enhancements with 1.4 x 10(-2) M beta-, hydroxypropyl-beta, and gamma-CD have been 1.9, 1.8 and 1.4-fold. respectively, with 2.4 x 10(-2) M methyl-beta-CD it was 2.8-fold whilst no effect was observed with alpha-CD. The stability constants of the complexes at pH 12 have been determined from the solubility isotherms. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：487 / 492

页数：6

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