The intra-articular injection of adipose-derived stem cells decreases pain and reduces inflammation in knee osteoarthritis, with or without the addition of platelet-rich plasma also improves functionality

被引：14

作者：

Carvalho Schweich-Adami, Laynna ^{[1
,2
]}

da Silva, Roberto Antoniolli ^{[3
,4
]}

Menezes, Jovino Nogueira da Silva ^{[5
]}

Baranoski, Adrivanio ^{[1
,2
]}

Kassuya, Candida Aparecida Leite ^{[6
]}

Bernardi, Luana ^{[1
,2
]}

Juliano Oliveira, Rodrigo ^{[1
,2
,7
]}

Conceicao Milan Brochado Antoniolli-Silva, Andreia ^{[1
,2
,3
]}

机构：

[1] Univ Fed Mato Grosso UFMS, Hosp Univ Maria Aparecida Pedrossian HUMAP EBSERH, Terapia Celular Genet Toxicol CeTroGen, Ctr Estudos Celulas Tronco, Campo Grande, MS, Brazil

[2] Univ Fed Mato Grosso UFMS, Fac Med Dr Helio Mandetta FAMED, Programa Posgrad Saude & Desenvolvimento Regiao C, Campo Grande, MS, Brazil

[3] Univ Fed Mato Grosso UFMS, Fac Med Dr Helio Mandetta FAMED, Campo Grande, MS, Brazil

[4] Univ Fed Mato Grosso UFMS, Hosp Univ Maria Aparecida Pedrossian HUMAP EBSERH, Setor Ortopedia & Traumatol, Campo Grande, MS, Brazil

[5] Hosp Canc Alfredo Abrao HCAA, Campo Grande, MS, Brazil

[6] Univ Fed Grande Dourados UFGD, Fac Ciencias Saude FCS, Dourados, MS, Brazil

[7] Univ Estadual Londrina UEL, Ctr Ciencias Biol CCB, Programa Pos grad Genet & Biol Mol, Londrina, Parana, Brazil

来源：

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE | 2022年 / 16卷 / 10期

关键词：

arthralgia; cell therapy; mesenchymal stem cells; regenerative medicine; ARTHROSCOPIC DEBRIDEMENT; CARTILAGE LESIONS;

D O I：

10.1002/term.3337

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The increase of individuals with Osteoarthritis (OA) has generated an increase in public spending in the treatments, which are still not that effective. So, the purpose of this study was to analyze and compare four types of interventions: platelet-rich plasma (PRP), adipose-derived stem cells (ADSCs), ADSCs + PRP and the standard surgical video arthroscopy (All groups passed through standard arthroscopy before intervention). The evaluation was performed by applying the questionnaires Western Ontario McMaster Universities, Short Form Health Survey 36 and Visual Analog Pain Scale, also by analyzing the synovial fluid (inflammatory cytokines, enzymatic, colorimetric and viscosity analysis), this evaluation happened in two moments: before the surgical procedures and after 6 months of the interventions and also was made a comparison to standard arthroscopy. The questionnaires results showed a greater improvement in the scores of the domains analyzed in the ADSCs + PRP group, followed by the ADSCs and PRP group. In the evaluation of inflammatory cytokines, there was a significant reduction in the cytokine IL-1b only in the ADSCs + PRP group (46%) and ADSCs (31%), of IL-6 in the ADSCs + PRP group (72%), of IL-8 in the ADSCs + PRP group (50%) and ADSCs (31%), and TNF in the ADSCs + PRP group (46%). There was also a significant increase in the amount of total proteins (79%) in the control group and polymorphonuclear cells (47%) in the ADSCs + PRP group. Taking all the results into account, we infer that therapies with ADSCs + PRP and only ADSCs are safe and effective over 6 months for the improvement of pain, functional capacity and joint inflammation in volunteers with OA. It is also considered that the use of ADSCs + PRP, particularly, is a promising alternative to help manage this disease, due to the better results presented among the four propose interventions.

引用

页码：900 / 912

页数：13