Genetic diversity of goats in East Java']Java through analysis of the cytochrome B and cytochrome oxidase I genes in mitochondrial DNA

被引：3

作者：

Suyadi, S. ^{[1
]}

Murtika, I. L. ^{[1
]}

Susilorini, T. E. ^{[1
]}

Septian, W. A. ^{[1
]}

Saputra, F. ^{[2
]}

Furqon, A. ^{[1
]}

机构：

[1] Brawijaya Univ, Fac Anim Sci, Malang 65145, East Java, Indonesia

[2] Indonesian Res Inst Anim Prod, Bogor 16002, West Java, Indonesia

来源：

SMALL RUMINANT RESEARCH | 2022年 / 209卷

关键词：

Indonesian goat; Haplotype; PCR; Sequencing; Evaluation;

D O I：

10.1016/j.smallrumres.2022.106659

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The aim of this research was to evaluate Kacang, Senduro, PE, Boer, and Saanen goats through the mitochondrial cytochrome B (Cyt B) and cytochrome oxidase I (COI) genes. The Parameter estimates used in the present study were number of haplotypes (NHap), haplotype diversity (Hd), nucleotide diversity (Pi), conserved sites, variable sites, nucleotide composition and genetic distance. A total of 47 samples were used in the current study. Genomic DNA was extracted from blood samples. DNA was amplified using primers designed according to caprine genomic sequence in GenBank (KF952601). The results showed that the Cyt B gene had 64 variable sites and 20 haplotypes. The Hd and Pi values were 0.891 +/- 0.032 and 0.00552 +/- 0.00375, respectively. There were 29 variable sites and six haplotypes in the partial COI gene. The Hd and Pi values were 0.471 +/- 0.058 and 0.00155 +/- 0.00152, respectively. In conclusion, the genetic distance among East Java goat populations was close to each other, except for the Saanen goat that has the farthest distance.

引用

页数：5

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