Antibody response against plasmid-encoded toxin (Pet) and the protein involved in intestinal colonization (Pic) in children with diarrhea produced by enteroaggregative Escherichia coli

被引：20
作者：
Bellini, EM
Elias, WP
Gomes, TAT
Tanaka, TL
Taddei, CR
Huerta, R
Navarro-Garcia, F
Martinez, MB [1 ]
机构：
[1] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, Sao Paulo, Brazil
[2] Inst Butantan, Lab Especial Microbiol, Sao Paulo, Brazil
[3] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
[4] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, BR-05508900 Sao Paulo, Brazil
[5] CINVESTAV, IPN, Dept Cell Biol, Mexico City 14000, DF, Mexico
来源：
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2005年 / 43卷 / 02期
基金：
巴西圣保罗研究基金会;
关键词：
EAEC; Pet; Pic; antibody response; IgG; IgM; IgA; diarrhea; children;
D O I：
10.1016/j.femsim.2004.08.008
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Enteroaggregative Escherichia eoli (EAEC) is an emerging cause of pediatric and adult travellers diarrhea. The mechanism by which EAEC induce diarrhea is not completely known. Two serine protease autotransporter proteins, named Pet and Pic have been identified in EAEC strains. Pet has enterotoxic and cytotoxic activities, while the role of Pic in pathogenesis may lie on its mucinolytic activity. Little is known about Pet and Pic biological activities in vivo. In this study the antibody responses against these autotransporter proteins in convalescent children is investigated. Fifteen (83%) children showed specific antibodies against Pet or Pic in their sera. IgG and IgM antibodies were the main isotype found. Specific antibodies against Pic, but not against Pet, were detected in sera from age-matched control group. These data show that specific anti-Pet and anti-Pic antibodies are produced during the course of a natural EAEC infection in children. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：259 / 264
页数：6
相关论文
共 29 条
[1]  
[Anonymous], SYSTEMATIC BOT MONOG
[2]   A SENSITIVE AND SPECIFIC DNA PROBE TO IDENTIFY ENTEROAGGREGATIVE ESCHERICHIA-COLI, A RECENTLY DISCOVERED DIARRHEAL PATHOGEN [J].
BAUDRY, B ;
SAVARINO, SJ ;
VIAL, P ;
KAPER, JB ;
LEVINE, MM .
JOURNAL OF INFECTIOUS DISEASES, 1990, 161 (06) :1249-1251
[3]   Enteroaggregative Escherichia coli associated with an outbreak of diarrhoea in a neonatal nursery ward [J].
Cobeljic, M ;
MiljkovicSelimovic, B ;
PaunovicTodosijevic, D ;
Velickovic, Z ;
Lepsanovic, Z ;
Zec, N ;
Savic, D ;
Ilic, R ;
Konstantinovic, S ;
Jovanovic, B ;
Kostic, V .
EPIDEMIOLOGY AND INFECTION, 1996, 117 (01) :11-16
[4]   ADHESIVE FACTOR FOUND IN STRAINS OF ESCHERICHIA-COLI BELONGING TO THE TRADITIONAL INFANTILE ENTEROPATHOGENIC SEROTYPES [J].
CRAVIOTO, A ;
GROSS, RJ ;
SCOTLAND, SM ;
ROWE, B .
CURRENT MICROBIOLOGY, 1979, 3 (02) :95-99
[5]   Combinations of putative virulence markers in typical and variant enteroaggregative Escherichia coli strains from children with and without diarrhoea [J].
Elias, WP ;
Uber, AP ;
Tomita, SK ;
Trabulsi, LR ;
Gomes, TAT .
EPIDEMIOLOGY AND INFECTION, 2002, 129 (01) :49-55
[6]   Pet, an autotransporter enterotoxin from enteroaggregative Escherichia coli [J].
Eslava, C ;
Navarro-García, F ;
Czeczulin, JR ;
Henderson, IR ;
Cravioto, A ;
Nataro, JP .
INFECTION AND IMMUNITY, 1998, 66 (07) :3155-3163
[7]  
Gomes TAT, 1998, J CLIN MICROBIOL, V36, P3609, DOI 10.1128/JCM.36.12.3609-3613.1998
[8]   PREVALENCE OF ESCHERICHIA-COLI STRAINS WITH LOCALIZED, DIFFUSE, AND AGGREGATIVE ADHERENCE TO HELA-CELLS IN INFANTS WITH DIARRHEA AND MATCHED CONTROLS [J].
GOMES, TAT ;
BLAKE, PA ;
TRABULSI, LR .
JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (02) :266-269
[9]  
GOMES TAT, 1991, J INFECT DIS, V164, P331, DOI 10.1093/infdis/164.2.331
[10]   INTESTINAL IMMUNE-RESPONSE OF VOLUNTEERS INGESTING A STRAIN OF ENTEROADHERENT (HEP-2 CELL-ADHERENT) ESCHERICHIA-COLI [J].
GOMEZ, HF ;
MATHEWSON, JJ ;
JOHNSON, PC ;
DUPONT, HL .
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 1995, 2 (01) :10-13
123