Impedance analysis of phosphatidylcholine-cholesterol system in bilayer lipid membranes

被引：33
作者：
Naumowicz, M
Petelska, AD
Figaszewski, ZA
机构：
[1] Univ Bialystok, Inst Chem, PL-15443 Bialystok, Poland
[2] Univ Warsaw, Fac Chem, Lab Electrochem Power Sources, PL-02093 Warsaw, Poland
来源：
ELECTROCHIMICA ACTA | 2005年 / 50卷 / 10期
关键词：
impedance spectroscopy; phosphatidylcholine; cholesterol; complex formation;
D O I：
10.1016/j.electacta.2004.09.023
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
Electrochemical impedance spectroscopy was used for the study of two-component lipid membranes. Phosphatidylcholine (lecithin, PC) and cholesterol (Ch) were chosen for the study because they are present in biological membranes and they fulfil essential functions in lively organisms. The theory of equilibrium between components of the membranes has been developed in order to obtain the parameters describing the PC-Ch complex. The equilibrium between the PC, Ch and PC-Ch complex on the basis of derived equations was considered and forming the 1:1 complex was proved. The following parameters of the complex were determined: capacitance (C-3) conductance (R-3(-1)), area occupied by complex molecule (A(3)) as well as stability constant of the complex (K). (C) 2004 Elsevier Ltd. All rights reserved.
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页码：2155 / 2161
页数：7
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