Anti-inflammatory and antioxidative properties of helicid protect against CCl4 induced acute liver injury in mice

Acute liver injury can be caused by chemicals and can lead to liver failure. We investigated the protective effect of helicid (HEL) on acute liver injury caused by CCl4 in mice. We found that ALT and AST levels as well as hepatic pathological damage in mice treated with CCl4 was increased significantly, while the effects were decreased by HEL treatment. HEL treatment increased the activity of T-SOD, GSH and CAT and reduced the level of MDA in CCl4 treated mice. HEL improved the histopathology of liver caused by CCl4. HEL also reduced TNF-alpha, IL-1 beta and IL- 6 activity caused by CCl4. We investigated the phosphorylation of p65 and I kappa B protein and found that HEL can alleviate liver damage via the NF-kappa B signaling pathway. Our findings indicate that HEL protects against acute liver injury induced by CCl4. The protective effect of HEL appears to be due to its antioxidative and anti-inflammatory properties through the NF-kappa B signaling pathway.