Optimization of a pravastatin quantification method using HPLC with ultraviolet detection in human serum for monitoring dyslipidemic patients

被引：4

作者：

Campos-Lara, Maria ^{[2
]}

Pinto-Almazan, Rodolfo ^{[2
]}

Oropeza, Martha V. ^{[2
]}

Mendoza-Espinoza, Jose Alberto ^{[1
]}

机构：

[1] Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Secc Externa Farmacol, CINVESTAV PIN, Mexico City 07000, DF, Mexico

[2] Inst Mexicano Seguro Social, Ctr Med Nacl SXXI, Unidad Invest Med & Farmacol, Mexico City, DF, Mexico

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2008年 / 31卷 / 05期

关键词：

pravastatin; serum levels; dyslipidemic patients;

D O I：

10.1080/10826070701853784

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high performance liquid chromatography (HPLC) method for the estimation of pravastatin in human serum samples has been developed to monitor dyslipidemic patients. The method was fully validated and validation parameters were in the linearity range 10-200ng/mL, correlation coefficient 0.99, mean recovery 0.73, quantification limit 10ng/mL, and limit of detection, 5ng/mL; this method was applied for pravastatin determination in human serum from Mexican dyslipidemic patients. Pravastatin values found for three studied patients were 73, 57, and 10ng/mL, indicating the importance of monitoring, due to the metabolic variability of these chronic patients.

引用

页码：667 / 674

页数：8

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