Partially Coherent Illumination Based Point-Diffraction Digital Holographic Microscopy Study Dynamics of Live Cells

被引：14
作者：
Zhuo, Kequn [1 ]
Wang, Yu [1 ]
Wang, Yang [1 ]
Wen, Kai [1 ]
Liu, Min [1 ]
Ma, Ying [1 ,2 ]
Zheng, Juanjuan [1 ,2 ,3 ]
Gao, Peng [1 ]
机构：
[1] Xidian Univ, Sch Phys & Optoelectron, Xian, Peoples R China
[2] Xidian Univ, Acad Adv Interdisciplinary Res, Xian, Peoples R China
[3] Chinese Acad Sci, Xian Inst Optic & Precis Mech, State Key Lab Transient Optic & Photon, Xian, Peoples R China
来源：
FRONTIERS IN PHYSICS | 2021年 / 9卷
基金：
中国国家自然科学基金;
关键词：
quantitative phase microscopy (QPM); digital holographic microscopy (DHM); point-diffraction; partially coherent illumination; live-cell imaging; SPATIAL COHERENCE; PHASE MICROSCOPY; INTERFEROMETRY;
D O I：
10.3389/fphy.2021.796935
中图分类号：
O4 [物理学];
学科分类号：
0702 ;
摘要：
This paper presents a partially coherent point-diffraction digital holographic microscopy (PC-pDHM) prototype and demonstrates its application in label-free imaging of the dynamic processes of live cells. In PC-pDHM, the light scattered by a rotating diffuser is coupled into a multi-mode fiber, and the output light is used as the partially coherent illumination (PCI), which reduces the speckle noise significantly in PC-pDHM. A polarization-grating is used to remold the object and the reference waves, and the fringe contrast of the generated hologram can be adjusted by changing the polarization of the illumination wave. Using the PC-pDHM prototype, transparent samples and notably the dynamic processes of live cells were imaged with high contrast and in a label-free manner, discovering the pathological mechanisms of biology in the cellular and sub-cellular levels.
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页数：7
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