A 6 x 6 drop plate method for simultaneous colony counting and MPN enumeration of Campylobacter jejuni, Listeria monocytogenes, and Escherichia coli

被引：327

作者：

Chen, CY ^{[1
]}

Nace, GW ^{[1
]}

Irwin, PL ^{[1
]}

机构：

[1] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2003年 / 55卷 / 02期

关键词：

Campylobacter; drop plate; enumeration; Listeria; MPN (most probable number); spiral plating;

D O I：

10.1016/S0167-7012(03)00194-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A protocol was developed using 96-well plates and multichannel pipettes for serial dilutions, followed by drop plating on agar in a 6 x 6 format. This protocol permits simultaneous plating of six dilutions which greatly decreases the number of plates utilized thereby saving incubator space for organisms such as Campylobacter which require unique environmental conditions. Published by Elsevier B.V.

引用

页码：475 / 479

页数：5