Existence of SARS-CoV-2 RNA on ambient particulate matter samples: A nationwide study in Turkey

被引：38

作者：

Kayalar, Ozgecan ^{[1
]}

Ari, Akif ^{[2
]}

Babuccu, Gizem ^{[3
]}

Konyalilar, Nur ^{[1
]}

Dogan, Ozlem ^{[3
]}

Can, Fusun ^{[3
]}

Sahin, Ulku A. ^{[4
]}

Gaga, Eftade O. ^{[5
]}

Kuzu, S. Levent ^{[6
]}

Ari, Pelin Erturk ^{[2
]}

Odabasi, Mustafa ^{[7
]}

Tasdemir, Yucel ^{[8
]}

Cindoruk, S. Siddik ^{[8
]}

Esen, Fatma ^{[8
]}

Sakin, Egemen ^{[8
]}

Caliskan, Burak ^{[8
]}

Tecer, Lokman H. ^{[9
]}

Ficici, Merve ^{[9
]}

Altin, Ahmet ^{[10
]}

Onat, Burcu ^{[4
]}

Ayvaz, Coskun ^{[4
]}

Uzun, Burcu ^{[4
]}

Saral, Arslan ^{[6
]}

Dogeroglu, Tuncay ^{[5
]}

Malkoc, Semra ^{[5
]}

Uzmez, Ozlem Ozden ^{[5
]}

Kunt, Fatma ^{[11
]}

Aydin, Senar ^{[11
]}

Kara, Melik ^{[7
]}

Yaman, Baris ^{[7
]}

Dogan, Guray ^{[12
]}

Olgun, Bihter ^{[12
]}

Dokumaci, Ebru N. ^{[12
]}

Gullu, Gulen ^{[13
]}

Uzunpinar, Elif S. ^{[14
]}

Bayram, Hasan ^{[1
,15
]}

机构：

[1] Koc Univ Res Ctr Translat Med KUTTAM, Istanbul, Turkey

[2] Abant Izzet Baysal Univ, Fac Engn, Dept Environm Engn, Golkoy Campus, Bolu, Turkey

[3] Koc Univ, Sch Med, Koc Univ Res Ctr Infect Dis, Dept Med Microbiol, Istanbul, Turkey

[4] Istanbul Univ Cerrahpasa, Fac Engn, Dept Environm Engn, Istanbul, Turkey

[5] Eskisehir Tech Univ, Fac Engn, Dept Environm Engn, Eskisehir, Turkey

[6] Yildiz Tech Univ, Fac Civil Engn, Dept Environm Engn, Istanbul, Turkey

[7] Dokuz Eylul Univ, Dept Environm Engn, Izmir, Turkey

[8] Bursa Uludag Univ, Fac Engn, Dept Environm Engn, Bursa, Turkey

[9] Namik Kemal Univ, Corlu Fac Engn, Dept Environm Engn, Tekirdag, Turkey

[10] Zonguldak Bulent Ecevit Univ, Dept Environm Engn, Zonguldak, Turkey

[11] Necmettin Erbakan Univ, Dept Environm Engn, Konya, Turkey

[12] Akdeniz Univ, Dept Environm Engn, Antalya, Turkey

[13] Hacettepe Univ, Dept Environm Engn, Ankara, Turkey

[14] Middle East Tech Univ, Dept Environm Engn, Ankara, Turkey

[15] Koc Univ, Sch Med, Dept Pulm Med, Istanbul, Turkey

来源：

SCIENCE OF THE TOTAL ENVIRONMENT | 2021年 / 789卷

关键词：

SARS-CoV-2; Particulate matter; Airborne; 3D-dPCR; INFLUENZA-VIRUS; COVID-19; POLLUTANTS; URBAN; PM2.5;

D O I：

10.1016/j.scitotenv.2021.147976

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Coronavirus disease 2019 (COVID-19) is caused by the SARS-CoV-2 virus and has been affecting the world since the end of 2019. The disease led to significant mortality and morbidity in Turkey, since the first case was reported on March 11th, 2020. Studies suggest a positive association between air pollution and SARS-CoV-2 infection. The aim of the present study was to investigate the role of ambient particulate matters (PM), as potential carriers for SARS-CoV-2. Ambient PM samples in various size ranges were collected from 13 sites including urban and urban-background locations and hospital gardens in 10 cities across Turkey between 13th of May and 14th of June 2020 to investigate the possible presence of SARS-CoV-2 RNA on ambient PM. A total of 203 daily samples (TSP, n = 80; PM2.5, n = 33; PM2.5-10, n = 23: PM10 mu m, n = 19; and 6 size segregated PM, n = 48) were collected using various samplers. The N1 gene and RdRP gene expressions were analyzed for the presence of SARS-CoV-2, as suggested by the Centers for Disease Control and Prevention (CDC). According to real time (RT)-PCR and three-dimensional (3D) digital (d) PCR analysis, dual RdRP and NI gene positivity were detected in 20 (9.8%) samples. Ambient PM-bound SARS-CoV-2 was analyzed quantitatively and the air concentrations of the virus ranged from 0.1 copies/m(3) to 23 copies/m(3). The highest percentages of virus detection on PM samples were from hospital gardens in Tekirdag, Zonguldak, and Istanbul, especially in PM2.5 mode. Findings of this study have suggested that SARS-CoV-2 may be transported by ambient partides, especially at sites close to the infection hot-spots. However. whether this has an impact on the spread of the virus infection remains to be determined. (C) 2021 Elsevier B.V. All rights reserved.

引用

页数：9

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