Synthesis and evaluation of fluorescent probes for the detection of calpain activity

被引：47
作者：
Mittoo, S
Sundstrom, LE
Bradley, M [1 ]
机构：
[1] Univ Southampton, Dept Chem, Southampton SO17 1BJ, Hants, England
[2] Univ Southampton, Dept Clin Neurosci, Southampton SO16 7PX, Hants, England
来源：
ANALYTICAL BIOCHEMISTRY | 2003年 / 319卷 / 02期
基金：
英国医学研究理事会;
关键词：
calpain I; FRET; fluorescence; proteolytic activity;
D O I：
10.1016/S0003-2697(03)00324-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Two new probes for the detection of calpain I activity based on fluorescence resonance energy transfer technology have been synthesized and evaluated. The probes incorporated the cleavage site present in alpha-spectrin, a naturally occurring substrate of calpain I. The design of the internally quenched substrates is such that the calpain-sensitive bond of the peptides (between the Tyr-Gly residues) is located centrally between the donor and the quencher chromophores. The calpain assay protocol is capable of detecting enzymatic activity in the nanomolar region. (C) 2003 Elsevier Science (USA). All rights reserved.
引用
收藏
页码：234 / 238
页数：5
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