Molecular cloning and expression of Xenopus laevis Requiem cDNA

被引：3
作者：
Konishi, M
Hiraoka, Y
Ogawa, M
Sakai, Y
Ishii, H
Aiso, S
机构：
[1] Keio Univ, Sch Med, Dept Anat, Shinjuku Ku, Tokyo 1608582, Japan
[2] Keio Univ, Sch Med, Dept Internal Med, Shinjuku Ku, Tokyo 1608582, Japan
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1999年 / 1445卷 / 01期
关键词：
apoptosis; embryo; Kruppel; Requiem; zinc-finger;
D O I：
10.1016/S0167-4781(99)00031-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Requiem is an apoptosis-associated gene, which was originally identified in mouse (T.G. Gabig et al., J. Biol. Chem. 269 (1994) 29515-29519). In this study, we isolated five independent cDNA clones for frog Requiem (xReq) from Xenopus laevis ovary. Sequence analysis of the multiple cDNAs has suggested that Xenopus genome contains at least two non-allelic copies of the xReq gene. The amino acid sequence deduced from the cDNAs contained a single Kruppel-type zinc-finger motif and two PHD-finger motifs. Northern analysis revealed that the ovary expressed xReq transcripts with different sizes. (C) 1999 Elsevier Science B.V. All rights reserved.
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收藏
页码：172 / 176
页数：5
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