Novel bioactive porous starch-siloxane matrix for bone regeneration: Physicochemical, mechanical, and in vitro properties

被引:28
作者
Aidun, Amir [1 ,2 ]
Zamanian, Ali [3 ,4 ]
Ghorbani, Farnaz [3 ,4 ,5 ,6 ]
机构
[1] Pasteur Inst Iran, Natl Cell Bank Iran, Tehran, Iran
[2] USERN, TBRG, Tehran, Iran
[3] Mat & Energy Res Ctr, Dept Nanotechnol & Adv Mat, Biomat Res Grp, Tehran, Iran
[4] Univ Tehran Med Sci, Skin & Stem cell Res Ctr, Tehran, Iran
[5] Islamic Azad Univ, Tehran Sci & Res Branch, Dept Biomed Engn, Tehran, Iran
[6] Aprin Adv Technol Dev Co, Dept Biomat, Tehran, Iran
关键词
bioactivity; biomaterials; GPTMS; silica; starch; tissue regeneration; PORE-SIZE; OSTEOGENIC DIFFERENTIATION; GELATIN SCAFFOLDS; FLOW PERFUSION; TISSUE; HYBRIDS; CELLS; CYTOCOMPATIBILITY; MICROSPHERES; BIOMATERIALS;
D O I
10.1002/bab.1694
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
So far, many studies have focused on biodegradable scaffolds for tissue engineering purposes. Herein, a starch-based biodegradable scaffold was fabricated by the freeze-drying method and cross-linked using a different concentration of 3-glycidoxypropyl-trimethoxysilane (GPTMS). Field emission scanning electron microscopy (FE-SEM) micrographs indicated an interconnected porous microstructure in which the porosity decreased as a function of starch and GPTMS content. Increasing the mechanical stability and decreasing absorption capacity and biodegradation ratio affected by the higher concentration of cross-linker and the changes in structure as a function of cross-linker enhancement. Moreover, the mineralization of hybrid structures in simulated body fluid was proved by FE-SEM image and X-ray diffraction analysis. Results indicated the more GPTMS in scaffolds led to more hydroxyapatite formation. The ability of the growth and proliferation of bone marrow mesenchyme stem cells on the constructs confirmed the ability of scaffolds for bone tissue engineering applications. (C) 2018 International Union of Biochemistry and Molecular Biology, Inc.
引用
收藏
页码:43 / 52
页数:10
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