Symplasmic phloem unloading and post-phloem transport during bamboo internode elongation

被引:23
作者
Deng, Lin [1 ]
Li, Pengcheng [1 ]
Chu, Caihua [1 ]
Ding, Yulong [2 ]
Wang, Shuguang [1 ]
机构
[1] Southwest Forestry Univ, Key Lab Sympodial Bamboo Res, Bailong Rd, Kunming 650224, Yunnan, Peoples R China
[2] Nanjing Forestry Univ, Bamboo Res Inst, Longpan Rd, Nanjing 210037, Jiangsu, Peoples R China
基金
国家重点研发计划;
关键词
Fargesia yunnanensis; internode elongation; phloem unloading; post-phloem transport; sucrose metabolism; water transport; SUCROSE-METABOLIZING ENZYMES; HYDRAULIC CONDUCTIVITY; DEVELOPMENTAL-CHANGES; STOMATAL CONDUCTANCE; SUGAR ACCUMULATION; VASCULAR BUNDLES; STRESS TOLERANCE; SWEET SORGHUM; SINK LEAVES; WATER;
D O I
10.1093/treephys/tpz140
中图分类号
S7 [林业];
学科分类号
0829 ; 0907 ;
摘要
In traditional opinions, no radial transportation was considered to occur in the bamboo internodes but was usually considered to occur in the nodes. Few studies have involved the phloem unloading and post-phloem transport pathways in the rapid elongating bamboo shoots. Our observations indicated a symplastic pathway in phloem unloading and post-unloading pathways in the culms of Fargesiayunnanensis Hsueh et Yi, based on a 5,6-carboxyfluorescein diacetate tracing experiment. Significant lignification and suberinization in fiber and parenchyma cell walls in maturing internodes blocked the apoplastic transport. Assimilates were transported out of the vascular bundles in four directions in the inner zones but in two directions in the outer zones via the continuum of parenchyma cells. In transverse sections, assimilates were outward transported from the inner zones to the outer zones. Assimilates transport velocities varied with time, with the highest values at 0):00 h, which were affected by water transport. The assimilate transport from the adult culms to the young shoots also varied with the developmental degree of bamboo shoots, with the highest transport velocities in the rapidly elongating internodes. The localization of sucrose, glucose, starch grains and the related enzymes reconfirmed that the parenchyma cells in and around the vascular bundles constituted a symplastic pathway for the radial transport of sugars and were the main sites for sugar metabolism. The parenchyma cells functioned as the 'rays' for the radial transport in and between vascular bundles in bamboo internodes. These results systematically revealed the transport mechanism of assimilate and water in the elongating bamboo shoots.
引用
收藏
页码:391 / 412
页数:22
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