Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats

被引：11

作者：

Shuralev, Eduard ^{[1
]}

Quinn, Padraig ^{[1
]}

Doyle, Mairead ^{[2
]}

Duignan, Anthony ^{[3
]}

Kwok, Hang Fai ^{[4
]}

Bezos, Javier ^{[5
]}

Olwill, Shane A. ^{[4
]}

Gormley, Eamonn ^{[2
]}

Aranaz, Alicia ^{[5
]}

Good, Margaret ^{[3
]}

Davis, William C. ^{[6
]}

Clarke, John ^{[1
]}

Whelan, Clare ^{[1
]}

机构：

[1] Enfer Sci, Unit T, Naas, Kildare, Ireland

[2] Univ Coll Dublin, Sch Agr Food Sci & Vet Med, Dublin 2, Ireland

[3] Dept Agr Fisheries & Food, Dublin, Ireland

[4] Fus Antibodies Ltd, Belfast BT17 0QL, Antrim, North Ireland

[5] Univ Complutense Madrid, Ctr Vigilancia Sanitaria Vet VISAVET, E-28040 Madrid, Spain

[6] Washington State Univ, Coll Vet Med, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA

来源：

VETERINARY MICROBIOLOGY | 2012年 / 154卷 / 3-4期

关键词：

Serology; Multiplex; Mycobacterium bovis; Tuberculosis; Goats; Antigens; LINKED-IMMUNOSORBENT-ASSAY; SEROLOGICAL DIAGNOSIS; CATTLE; TUBERCULOSIS; PARATUBERCULOSIS; TESTS; IMMUNOASSAY; ANTIGEN; AVIUM;

D O I：

10.1016/j.vetmic.2011.07.028

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A study was conducted to optimise a multiplex serological immunoassay for use in identification of goats infected with Mycobacterium bovis. To assess assay specificity, 31 goats with a history of being free from M. bovis infection were used. To determine assay sensitivity, 180 Single Intradermal Comparative Tuberculin test (SICTT) positive goats were recruited. Additionally, 286 SICTT negative goats classed as potentially exposed animals present in the same positive herds were also included in the study. The results of the assay demonstrated a specificity of 100%. The multiplex assay detected 57/60 SIC (95.0%) positive animals in one M. bovis infected herd and 120/120(100%) in a second herd. In a separate experiment, 28 M. caprae culture confirmed infected goats from Spain were assayed, of which 24 (85.7%) were found positive in the test. The results show that inclusion of an antibody based assay can improve the ability to identify M. bovis and M. caprae infected goats. With further development and validation the multiplex assay may prove to be a useful tool for control of M. bovis and M. caprae infection in goats. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：292 / 297

页数：6

共 26 条

[1]

Acosta B, 2000, AUST VET J, V78, P423

[2] Interference of paratuberculosis with the diagnosis of tuberculosis in a goat flock with a natural mixed infection [J].