Immobilization of Peroxidase onto Magnetite Modified Polyaniline

被引：10

作者：

Barbosa, Eduardo Fernandes ^{[2
]}

Javier Molina, Fernando ^{[3
]}

Lopes, Flavio Marques ^{[1
,2
]}

Antonio Garcia-Ruiz, Pedro ^{[3
]}

Caramori, Samantha Salomao ^{[1
]}

Fernandes, Katia Flavia ^{[2
]}

机构：

[1] Univ Estadual Goias, Unidade Univ Ciencias Exatas & Tecnol, BR-75132903 Anapolis, Go, Brazil

[2] Univ Estadual Goias, Inst Ciencias Biol, Lab Quim Prot, BR-74001970 Goiania, Go, Brazil

[3] Univ Murcia, Dept Quim Organ, Grp Quim Carboydratos & Biotecnol Alimentos, Murcia 34100, Spain

来源：

SCIENTIFIC WORLD JOURNAL | 2012年

关键词：

HORSERADISH-PEROXIDASE; TRYPSIN IMMOBILIZATION; HYDROGEN-PEROXIDE; BIOSENSOR; POLYMER; COMPOSITE; ENZYME;

D O I：

10.1100/2012/716374

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The present study describes the immobilization of horseradish peroxidase (HRP) on magnetite-modified polyaniline (PANImG) activated with glutaraldehyde. After the optimization of the methodology, the immobilization of HRP on PANImG produced the same yield (25%) obtained for PANIG with an efficiency of 100% (active protein). The optimum pH for immobilization was displaced by the effect of the partition of protons produced in the microenvironment by the magnetite. The tests of repeated use have shown that PANImG-HRP can be used for 13 cycles with maintenance of 50% of the initial activity.

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页数：5

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