Preclinical study for the treatment of diabetes mellitus using β-like cells derived from human dental pulp stem cells

被引：2
|
作者：
Inada, Ryo [1 ]
Mendoza, Hiromi Yagi [2 ]
Tanaka, Tomoko [2 ]
Horie, Tetsuro [2 ]
Satomi, Takafumi [1 ]
机构：
[1] Nippon Dent Univ Tokyo, Dept Oral & Maxillofacial Surg, Sch Life Dent Tokyo, Chiyoda Ku, 1-9-20 Fujimi, Tokyo 1028159, Japan
[2] Nippon Dent Univ Tokyo, Dept Oral Hlth, Sch Life Dent Tokyo, Chiyoda Ku, 1-9-20 Fujimi, Tokyo 1028159, Japan
来源：
REGENERATIVE MEDICINE | 2022年 / 17卷 / 12期
关键词：
dental pulp; diabetes mellitus; mesenchymal stem cells; rats; HYDROGEN-SULFIDE; HUMAN TOOTH; C-KIT; DIFFERENTIATION;
D O I：
10.2217/rme-2022-0092
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Aim: The current study assessed whether insulin-producing cells obtained from dental pulp stem cells (DPSCs) can be a new therapeutic approach in a rat model of diabetes mellitus (DM). Materials & methods: Stem cells were differentiated into pancreatic beta cells under hydrogen sulfide exposure via 2D and 3D methods. Each beta-like cell was immunostained and transplanted into DM rats, after which the in vivo therapeutic effect was determined. Results: Immunostaining revealed the expression of various beta-cell markers in each beta-like cell differentiated using the 3D method. DPSC-derived beta-like cell differentiated via the 3D method promoted a sufficient therapeutic effect. Conclusion: The 3D method promoted islet differentiation, indicating that DPSC-derived beta-like cell transplantation could be a new approach for DM treatment.
引用
收藏
页码：905 / 913
页数：9
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