Cytochrome c oxidase subunit 1 gene reveals species composition and phylogenetic relationships of Oesophagostomum spp. infecting pigs in northeastern Brazil

被引：1

作者：

Alves Bacelar, Polyanna Araujo ^{[1
,2
]}

Lima Monteiro, Kerla Joeline ^{[1
,2
]}

Calegar, Deiviane Aparecida ^{[1
]}

dos Santos, Jessica Pereira ^{[1
,2
]}

Coronato-Nunes, Beatriz ^{[3
]}

Chaves dos Reis, Elis Regina ^{[4
]}

Boia, Marcio Neves ^{[5
]}

Jaeger, Lauren Hubert ^{[6
]}

Carvalho-Costa, Filipe Anibal ^{[1
]}

机构：

[1] Fundacao Oswaldo Cruz Fiocruz, Lab Epidemiol & Sistemat Mol, Inst Oswaldo Cruz, Rio De Janeiro, RJ, Brazil

[2] Fundacao Oswaldo Cruz Fiocruz, Escritorio Tecn Reg, Teresina, PI, Brazil

[3] Ctr Univ Arthur Sa Earp Neto UNIFASE, Fac Med Petropolis FMP, Petropolis, RJ, Brazil

[4] Secretaria Municipal Saude Nossa Senhora Nazare, Nossa Senhora Nazare, PI, Brazil

[5] Fundacao Oswaldo Cruz Fiocruz, Lab Biol & Parasitol Mamiferos Silvestres Reserva, Inst Oswaldo Cruz Fiocruz, Rio De Janeiro, RJ, Brazil

[6] Univ Fed Juiz de Fora UFJF, Fac Farm, Dept Ciencias Farmaceut, Juiz De Fora, MG, Brazil

来源：

REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA | 2022年 / 31卷 / 01期

关键词：

DNA barcode; Oesophagostomum; molecular taxonomy; pigs; PARASITIC HELMINTHS; DENTATUM;

D O I：

10.1590/S1984-29612022016

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Helminths of the genus Oesophagostomum cause enteric diseases and affect domestic animals such as pigs. The aim of this study was to explore the species composition and genetic diversity of Oesophagostomum spp. infecting pigs in close contact with humans in the state of Piaui, Brazil. Eighty-seven fecal samples were collected for parasitological tests and molecular analysis. Through microscopy, the overall positivity rate for strongyliform eggs was 81.6% among the pigs studied. Forty-two strongyliform egg samples were subjected to PCR and six cox1 sequences (637 bp) were identified for the genus Oesophagostomum. The sequences were identified as Oesophagostomum dentatum, O. quadrispinulatum and O. columbianum. In the phylogenetic tree and haplotype network, 89 sequences were separated into seven clusters, which also included reference sequences from GenBank. Oesophagostomum dentatum and O. quadrispinulatum were seen to be closely related species and formed a monophyletic group related to O. aculeatum. Oesophagostomum columbianum showed similarity with sequences from parasites infecting small ruminants and the clade was positioned closer to O. bifurcum. High interspecific diversity was found and intraspecific diversity varied according to the species. This was the first study to characterize Oesophagostomum DNA sequences obtained from pigs in Brazil.

引用

页数：13

共 33 条

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