Comparisons of competitive enzyme-linked immunosorbent assay and one step RT-PCR tests for the detection of Bluetongue virus in south west of Iran

被引：0

作者：

Momtaz, Hassan ^{[1
]}

Nejat, Shahin ^{[2
]}

Souod, Negar

Momeni, Monochehr ^{[3
]}

Safari, Sohrab ^{[3
]}

机构：

[1] Islamic Azad Univ, Fac Vet Med, Dept Microbiol, Shahrekord Branch, Shahrekord, Iran

[2] Islamic Azad Univ, Fac Vet Med, Dept Clin Sci, Shahrekord Branch, Shahrekord, Iran

[3] Islamic Azad Univ, Res Ctr Biotechnol, Shahrekord Branch, Shahrekord, Iran

来源：

AFRICAN JOURNAL OF BIOTECHNOLOGY | 2011年 / 10卷 / 36期

关键词：

Bluetongue virus; C-ELISA; RT-PCR; Sheep; Iran; POLYMERASE CHAIN-REACTION; UNITED-STATES; CULICOIDES-VARIIPENNIS; TISSUE SAMPLES; SHEEP FLOCKS; DIAGNOSIS; SEROTYPES; INFECTION; SEQUENCE; PROTEIN;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bluetongue is a noncontagious, arthropod-borne viral disease of both domestic and wild ruminants. Bluetongue virus (BTV) is the type of species of the genus Orbivirus within the family Reoviridae. BTV is endemic in some areas with cattle and wild ruminants serving as reservoirs for the virus. Clinical symptoms are often seen in sheep. There are several methods for the detection of Bluetongue virus, among them the molecular technique like RT-PCR is considered as the most sensitive and reliable one. The aim of this study was to comprise competitive enzyme-linked immunosorbent assay (C-ELISA) with one step RT-PCR test for the detection of BTV in sheep. A total of 770 blood samples were obtained from sheep (265 serum positive samples and 505 serum negative samples in C-ELISA). According to our data, out of the 265 serum positive samples in ELISA test, 234 were positive in RT-PCR assay whereas all serum negative samples were negative in RT-PCR experiment. According to the results, the PCR assay was more sensitive and reliable than ELISA technique for the diagnosis of Bluetongue virus.

引用

页码：6857 / 6862

页数：6

共 50 条

[11] Bluetongue virus diagnosis of clinical cases by a duplex reverse transcription-PCR: a comparison with conventional methods [J].

Billinis, C ;

Koumbati, M ;

Spyrou, V ;

Nomikou, K ;

Mangana, O ;

Panagiotidis, CA ;

Papadopoulos, O .

JOURNAL OF VIROLOGICAL METHODS, 2001, 98 (01) :77-89

[12] Performance evaluation of a competitive ELISA test used for Bluetongue antibody detection in France, a recently infected area [J].

Biteau-Coroller, Fabienne ;

Gerbier, Guillaume ;

Staerk, Katharina D. C. ;

Grillet, Colette ;

Albina, Emmanuel ;

Zientara, Stephan ;

Roger, Francois .

VETERINARY MICROBIOLOGY, 2006, 118 (1-2) :57-66

[13] Bluetongue virus in the French Island of Reunion [J].

Bréard, E ;

Sailleau, C ;

Hamblin, C ;

Zientara, S .

VETERINARY MICROBIOLOGY, 2005, 106 (3-4) :157-165

[14] The epidemiology and diagnosis of bluetongue with particular reference to Corsica [J].

Breard, E ;

Hamblin, C ;

Hammoumi, S ;

Sailleau, C ;

Dauphin, G ;

Zientara, S .

RESEARCH IN VETERINARY SCIENCE, 2004, 77 (01) :1-8

[15] Comparison of genome segments 2, 7 and 10 of bluetongue viruses serotype 2 for differentiation between field isolates and the vaccine strain [J].

Bréard, E ;

Sailleau, C ;

Coupier, H ;

Mure-Ravaud, K ;

Hammoumi, S ;

Gicquel, B ;

Hamblin, C ;

Dubourget, P ;

Zientara, S .

VETERINARY RESEARCH, 2003, 34 (06) :777-789

[16] Isolation and identification of bluetongue virus [J].

Clavijo, A ;

Heckert, RA ;

Dulac, GC ;

Afshar, A .

JOURNAL OF VIROLOGICAL METHODS, 2000, 87 (1-2) :13-23

[17]

Dadhich H, 2004, Vet Ital, V40, P564

[18] IDENTIFYING BLUETONGUE VIRUS-RIBONUCLEIC-ACID SEQUENCES BY THE POLYMERASE CHAIN-REACTION [J].

DANGLER, CA ;

DEMATTOS, CA ;

DEMATTOS, CC ;

OSBURN, BI .

JOURNAL OF VIROLOGICAL METHODS, 1990, 28 (03) :281-292

[19] A NEW BLUETONGUE VIRUS SEROTYPE ISOLATED IN KENYA [J].

DAVIES, FG ;

MUNGAI, JN ;

PINI, A .

VETERINARY MICROBIOLOGY, 1992, 31 (01) :25-32

[20]

Eaton B T, 2004, Vet Ital, V40, P525

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