AtSAP130/AtSF3b-3 Function is Required for Reproduction in Arabidopsis thaliana

被引：16
作者：
Aki, Shiori [1 ]
Nakai, Hideto [1 ]
Aoyama, Takashi [1 ]
Oka, Atsuhiro [1 ]
Tsuge, Tomohiko [1 ]
机构：
[1] Kyoto Univ, Inst Chem Res, Kyoto 6110011, Japan
来源：
PLANT AND CELL PHYSIOLOGY | 2011年 / 52卷 / 08期
基金：
日本学术振兴会;
关键词：
Arabidopsis; COP9 signalosome (CSN); Pollen development; SPLICEOSOME-ASSOCIATED PROTEIN 130 (SAP130); Splicing; Splicing factor 3b (SF3b); MALE GAMETOPHYTE DEVELOPMENT; CELL-CYCLE PROGRESSION; HUMAN STAGA COMPLEX; UBIQUITIN LIGASE; POLLEN DEVELOPMENT; BINDING-PROTEIN; DAMAGED DNA; U2; SNRNP; REVERSE GENETICS; RIBOSOMAL-RNA;
D O I：
10.1093/pcp/pcr077
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Flowering plants produce multicellular gametophytes through an elaborate regulation of gametogenesis. During female and male gametogenesis in Arabidopsis thaliana, sporogenous cells differentiate and undergo meiosis to produce megaspores and microspores, which in turn go through mitosis to develop into multicellular gametophytes. Here we report that the Arabidopsis spliceosomal protein, SPLICEOSOME-ASSOCIATED PROTEIN 130 (AtSAP130), is required for proper reproduction. AtSAP130 is encoded by two genes, AtSAP130a and AtSAP130b. Plants with reduced expression of the AtSAP130 genes, induced by RNA interference, showed a defect in fertilization. Besides functional impairment observed in the female reproductive organs, analysis focusing on pollen development revealed defects in the transition from the microspore to the bicellular stage. Our results suggest that AtSAP130a and AtSAP130b play an indispensable role in specific spatiotemporal events in reproduction.
引用
收藏
页码：1330 / 1339
页数：10
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