Identification and functional characterization of eight CYP3A4 protein variants

The genetic component of the inter-individual variability in CYP3A4 activity has been estimated to be between 60% and 90%, but the underlying genetic factors remain largely unknown. A study of 213 Middle and Western European DNA samples resulted in the identification of 18 new CYP3A4 variants, including eight protein variants, A total of 7.5% of the population studied was found to be heterozygous for one of these variants, in a bacterial heterologous expression system, two mutants, R130Q and P416L, did not result in detectable P450 holoprotein. One mutant, T363M, expressed at significantly lower levels than wild-type CYP3A4, G56D, V170I, D174H and M445T were not significantly different when compared with wild-type CYP3A4 in expression or steroid hydroxylase activity, L373F displayed a significantly altered testosterone metabolite profile and a four-fold increase in the K-m value for 1'-OH midazolam formation. The results suggest a limited contribution of CYP3A4 protein variants to the inter-individual variability of CYP3A4 activity in Caucasians, Some variants may, however, play a role in the atypical response to drugs or altered sensitivity to carcinogens. Pharmacogenetics 11:447-458 (C) 2001 Lippincott Williams & Wilkins.