Optogenetic investigation of neural circuits in vivo

被引：57

作者：

Carter, Matthew E. ^{[1
]}

de Lecea, Luis

机构：

[1] Stanford Univ, Neurosci Program, Stanford, CA 94305 USA

来源：

TRENDS IN MOLECULAR MEDICINE | 2011年 / 17卷 / 04期

关键词：

RESTORES VISUAL RESPONSES; LIGHT-DRIVEN PROTON; OPTICAL CONTROL; MILLISECOND-TIMESCALE; NOCICEPTIVE NEURONS; GENETIC DISSECTION; ECTOPIC EXPRESSION; DROSOPHILA LARVAE; REMOTE-CONTROL; CHANNELRHODOPSIN-2;

D O I：

10.1016/j.molmed.2010.12.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The recent development of light-activated optogenetic probes allows for the identification and manipulation of specific neural populations and their connections in awake animals with unprecedented spatial and temporal precision. This review describes the use of optogenetic tools to investigate neurons and neural circuits in vivo. We describe the current panel of optogenetic probes, methods of targeting these probes to specific cell types in the nervous system, and strategies of photostimulating cells in awake, behaving animals. Finally, we survey the application of optogenetic tools to studying functional neuroanatomy, behavior and the etiology and treatment of various neurological disorders.

引用

页码：197 / 206

页数：10

共 97 条

[61] Genetic dissection of neural circuits [J].

Luo, Liqun ;

Callaway, Edward M. ;

Svoboda, Karel .

NEURON, 2008, 57 (05) :634-660

[62] A robust and high-throughput Cre reporting and characterization system for the whole mouse brain [J].

Madisen, Linda ;

Zwingman, Theresa A. ;

Sunkin, Susan M. ;

Oh, Seung Wook ;

Zariwala, Hatim A. ;

Gu, Hong ;

Ng, Lydia L. ;

Palmiter, Richard D. ;

Hawrylycz, Michael J. ;

Jones, Allan R. ;

Lein, Ed S. ;

Zeng, Hongkui .

NATURE NEUROSCIENCE, 2010, 13 (01) :133-U311

[63] Channelrhodopsin-1: A light-gated proton channel in green algae [J].

Nagel, G ;

Ollig, D ;

Fuhrmann, M ;

Kateriya, S ;

Mustl, AM ;

Bamberg, E ;

Hegemann, P .

SCIENCE, 2002, 296 (5577) :2395-2398

[64] Channelrhodopsin-2, a directly light-gated cation-selective membrane channel [J].

Nagel, G ;

Szellas, T ;

Huhn, W ;

Kateriya, S ;

Adeishvili, N ;

Berthold, P ;

Ollig, D ;

Hegemann, P ;

Bamberg, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (24) :13940-13945

[65] Nanosculpting reversed wavelength sensitivity into a photoswitchable iGluR [J].

Numano, Rika ;

Szobota, Stephanie ;

Lau, Albert Y. ;

Gorostiza, Pau ;

Volgraf, Matthew ;

Roux, Benoit ;

Trauner, Dirk ;

Isacoff, Ehud Y. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (16) :6814-6819

[66] Substitution of 5-HT1A Receptor Signaling by a Light-activated G Protein-coupled Receptor [J].

Oh, Eugene ;

Maejima, Takashi ;

Liu, Chen ;

Deneris, Evan ;

Herlitze, Stefan .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (40) :30825-30836

[67] Channelrhodopsin-2-assisted circuit mapping of long-range callosal projections [J].

Petreanu, Leopoldo ;

Huber, Daniel ;

Sobczyk, Aleksander ;

Svoboda, Karel .

NATURE NEUROSCIENCE, 2007, 10 (05) :663-668

[68] The subcellular organization of neocortical excitatory connections [J].

Petreanu, Leopoldo ;

Mao, Tianyi ;

Sternson, Scott M. ;

Svoboda, Karel .

NATURE, 2009, 457 (7233) :1142-U100

[69] Seven-transmembrane receptors [J].

Pierce, KL ;

Premont, RT ;

Lefkowitz, RJ .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2002, 3 (09) :639-650

[70] Hypothalamic regulation of sleep and circadian rhythms [J].

Saper, CB ;

Scammell, TE ;

Lu, J .

NATURE, 2005, 437 (7063) :1257-1263

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