Classification, capsular PCR typing and genetic diversity of Pasteurella multocida isolated from sheep and goats in Iran using Bam HI, Hind III restriction endonuclease enzymes by PCR-RFLP

The aim of this study was to differentiate Pasteurella multocidafrom ovine and caprine isolates in Iran by PCR-RFLP generated by restriction endonuclease enzymes Bam HI and Hind III. Among the isolates, 92.3% was identified as capsular type A and 7.7% as type D. All P. multocidaisolates were found to be virulent except for two isolates that caused no death in mice bio-assay. Restriction endonuclease analysis revealed 7 distinct profiles by digestion with Hind III, and Bam HI. Strains of the same PCR-RFLP type were similar to each other, whereas strains with different PCR-RFLP types were diversified. The HnidIII was found more useful for differentiating between DNA fingerprints of P. multocida ovine and caprine isolates. The typing with PCR-RFLP and correspondence with MDT gave consistent results. The strains of type II were found to be more associated with diseases of animals. PCR-RFLP pattern could be used for identification, differentiation and classification of P. multocida.