A sensitive resonance Rayleigh light scattering method for alpinetin using gold nanorods probes

被引：4
作者：
Bi, Shuyun [1 ]
Sun, Xiaoyue [1 ]
Wang, Yu [1 ]
Wu, Jun [1 ]
Zhou, Huifeng [1 ]
机构：
[1] Changchun Normal Univ, Coll Chem, Changchun 130032, Jilin, Peoples R China
来源：
LUMINESCENCE | 2018年 / 33卷 / 07期
关键词：
alpinetin; Alpinia katsumadai; gold nanorods (AuNRs); resonance Rayleigh light scattering (RLS); NANOPARTICLES; DNA; CHROMATOGRAPHY; ASSEMBLIES; CARDAMONIN; SEPARATION; MECHANISM; CELLS; ASSAY; SHAPE;
D O I：
10.1002/bio.3531
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A sensitive resonance Rayleigh light scattering (RLS) assay for alpinetin was developed based on alpinetin-modified gold nanorods (AuNRs). Alpinetin could interact with AuNRs and formed a new assembly by electrostatic attraction. In pH7.4 Tris-HCl buffer solution, the assembly of alpinetin-AuNRs showed a sensitive RLS signal. Under optimum conditions, the magnitude of enhanced RLS intensity (I-RLS) was proportional to the concentration of alpinetin over the range 0.027-3.24gml(-1), with a detection limit of 1.79ngml(-1) (by 3 sigma). The developed RLS method was successfully applied to the detection of alpinetin in real or synthesized samples. Alpinetin recoveries were 90.4-108.7% with an RSD of 0.82-2.9% (n=5) for Alpinia katsumadai samples, and 95.1-103.7% with an RSD of 0.28-3.9% (n=5) for synthesized samples. The results showed that this new approach was convenient, reliable and sensitive.
引用
收藏
页码：1164 / 1170
页数：7
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