Flash pyrolysis - a rapid method for screening bacterial species for the presence of bacteriohopanepolyols

被引:16
作者
Sugden, MA [1 ]
Talbot, HM [1 ]
Farrimond, P [1 ]
机构
[1] Univ Newcastle Upon Tyne, Sch Civil Engn & Geosci, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England
基金
英国自然环境研究理事会;
关键词
D O I
10.1016/j.orggeochem.2004.12.003
中图分类号
P3 [地球物理学]; P59 [地球化学];
学科分类号
0708 ; 070902 ;
摘要
Flash pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) was applied to freeze dried cells of eleven bacterial species. Six species known to synthesise bacteriohopanepolyols (BHPs) were found to produce easily identifiable C-27-C-35 hopenes and hopanes upon flash pyrolysis. These compounds could not be observed in the pyrolysis products of five species, two of which are known not to produce BHPs. In the search for BHP-containing bacteria this rapid screening approach prevents unnecessary time-consuming laboratory work to characterise species that would produce negative results. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:975 / 979
页数:5
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