Metagenomic Next-Generation Sequencing of Bloodstream Microbial Cell-Free Nucleic Acid in Children With Suspected Sepsis in Pediatric Intensive Care Unit

被引:31
作者
Yan, Gangfeng [1 ]
Liu, Jing [1 ]
Chen, Weiming [1 ]
Chen, Yang [1 ]
Cheng, Ye [1 ]
Tao, Jinhao [1 ]
Cai, Xiaodi [1 ]
Zhou, Yang [2 ]
Wang, Yixue [1 ]
Wang, Mingbang [3 ]
Lu, Guoping [1 ]
机构
[1] Fudan Univ, Natl Childrens Med Ctr, Paediat Intens Care Unit, Childrens Hosp, Shanghai, Peoples R China
[2] BGI PathoGenesis Pharmaceut Technol Co Ltd, BGI Shenzhen, Shenzhen, Peoples R China
[3] Fudan Univ, Natl Childrens Med Ctr, Natl Hlth Commiss NHC,Key Lab Neonatal Dis, Shanghai Key Lab Birth Defects,Div Neonatol,Child, Shanghai, Peoples R China
基金
美国国家科学基金会;
关键词
bloodstream infection; metagenomic next-generation sequencing; pediatric intensive care unit; Pneumocystis jirovecii; sepsis; INFECTIONS; DIAGNOSIS; MANAGEMENT; ADULTS;
D O I
10.3389/fcimb.2021.665226
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Bloodstream infection is a life-threatening complication in critically ill patients. Multi-drug resistant bacteria or fungi may increase the risk of invasive infections in hospitalized children and are difficult to treat in intensive care units. The purpose of this study was to use metagenomic next-generation sequencing (mNGS) to understand the bloodstream microbiomes of children with suspected sepsis in a pediatric intensive care unit (PICU). mNGS were performed on microbial cell-free nucleic acid from 34 children admitted to PICU, and potentially pathogenic microbes were identified. The associations of serological inflammation indicators, lymphocyte subpopulations, and other clinical phenotypes were also examined. mNGS of blood samples from children in PICU revealed potential eukaryotic microbial pathogens. The abundance of Pneumocystis jirovecii was positively correlated with a decrease in total white blood cell count and immunodeficiency. Hospital-acquired pneumonia patients showed a significant increase in blood bacterial species richness compared with community-acquired pneumonia children. The abundance of bloodstream bacteria was positively correlated with serum procalcitonin level. Microbial genome sequences from potential pathogens were detected in the bloodstream of children with suspected sepsis in PICU, suggesting the presence of bloodstream infections in these children.
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页数:10
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