Candida tropicalis isolates obtained from veterinary sources show resistance to azoles and produce virulence factors

被引:55
作者
Cordeiro, Rossana de Aguiar [1 ]
de Oliveira, Jonathas Sales [1 ]
Collares Maia Castelo-Branco, Debora de Souza [1 ]
Cordeiro Teixeira, Carlos Eduardo [1 ]
de Farias Marques, Francisca Jakelyne [1 ]
Bittencourt, Paula Vago [1 ,2 ]
Carvalho, Vitor Luz [1 ]
Pinheiro Gomes Bandeira, Tereza de Jesus [1 ,3 ]
Nogueira Brilhante, Raimunda Samia [1 ]
Bezerra Moreira, Jose Luciano [1 ]
Pereira-Neto, Waldemiro de Aquino [1 ]
Costa Sidrim, Jose Julio [1 ]
Gadelha Rocha, Marcos Fabio [1 ,2 ]
机构
[1] Univ Fed Ceara, Postgrad Program Med Microbiol, Specialized Med Mycol Ctr, Fortaleza, Ceara, Brazil
[2] Univ Estadual Ceara, Postgrad Program Vet Sci, Sch Vet, Fortaleza, Ceara, Brazil
[3] Christus Coll UNICHRISTUS, Sch Med, Fortaleza, Ceara, Brazil
关键词
Candida tropicalis; susceptibility; antifungals; virulence factors; resistance; COCKATIELS NYMPHICUS-HOLLANDICUS; ANTIFUNGAL SUSCEPTIBILITY; BIOFILM PRODUCTION; PHOSPHOLIPASE; YEASTS; MECHANISMS; MICROBIOTA; IDENTIFICATION; ALBICANS;
D O I
10.1093/mmy/myu081
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Candida tropicalis has been associated with invasive candidiasis, being the first or second most common non-Candida albicans Candida species isolated in humans with candidemia and candiduria, as well as being frequently isolated from healthy animals. This study aimed to characterize C. tropicalis isolates (n = 64) obtained from several animal species regarding antifungal susceptibility and production of virulence factors. The isolates were obtained from the microbiota of healthy animals (goats, n = 25; sheep, n = 6; psittacines, n = 14; rheas, n = 6; horses, n = 2; sirenians, n = 5; shrimp, n = 1), as well as from aquatic mammals found dead in the environment (cetaceans, n = 5). The isolates were subjected to in vitro susceptibility testing by broth microdilution according to the CLSI M27-A3 protocol against amphotericin B, caspofungin, itraconazole, and fluconazole. We also evaluated the virulence attributes, such as proteases and phospholipases, as well as biofilm formation. Resistance to itraconazole (n = 29) and fluconazole (n = 30) was detected among isolates from every source; resistance to both azoles was detected in 24 isolates, but none of them were resistant to amphotericin B and caspofungin. Protease production was detected in the majority of the isolates (n = 59), but phospholipase was produced by only a few of them (n = 6). The isolates showed different patterns in biofilm production, being considered strong producers (n = 41), moderate producers (n = 11), weak producers (n = 9) or non-producers (n = 3). In summary, C. tropicalis isolated from animals showed high rate of resistance to azoles, expressed virulence factors and therefore may represent a potential threat to human and animal health.
引用
收藏
页码:145 / 152
页数:8
相关论文
共 46 条
[1]   EXTRACELLULAR PROTEOLYTIC ACTIVITY OF CRYPTOCOCCUS-NEOFORMANS [J].
AOKI, S ;
ITOKUWA, S ;
NAKAMURA, K ;
KATO, J ;
NINOMIYA, K ;
VIDOTTO, V .
MYCOPATHOLOGIA, 1994, 128 (03) :143-150
[2]   Candida peritonitis in dogs: report of 5 cases [J].
Bradford, Katy ;
Meinkoth, Jim ;
McKeirnen, Kelci ;
Love, Brenda .
VETERINARY CLINICAL PATHOLOGY, 2013, 42 (02) :227-233
[3]   Characterization of the gastrointestinal yeast microbiota of cockatiels (Nymphicus hollandicus): a potential hazard to human health [J].
Brilhante, R. S. N. ;
Castelo-Branco, D. S. C. M. ;
Soares, G. D. P. ;
Astete-Medrano, D. J. ;
Monteiro, A. J. ;
Cordeiro, R. A. ;
Sidrim, J. J. C. ;
Rocha, M. F. G. .
JOURNAL OF MEDICAL MICROBIOLOGY, 2010, 59 (06) :718-723
[4]   Yeasts from Macrobrachium amazonicum: a focus on antifungal susceptibility and virulence factors of Candida spp. [J].
Brilhante, Raimunda S. N. ;
Paiva, Manoel A. N. ;
Sampaio, Celia M. S. ;
Teixeira, Carlos E. C. ;
Castelo-Branco, Debora S. C. M. ;
Leite, Joao J. G. ;
Moreira, Camila A. ;
Silva, Liliane P. ;
Cordeiro, Rossana A. ;
Monteiro, Andre J. ;
Sidrim, Jose J. C. ;
Rocha, Marcos F. G. .
FEMS MICROBIOLOGY ECOLOGY, 2011, 76 (02) :268-277
[5]   The anatomical distribution and antimicrobial susceptibility of yeast species isolated from healthy dogs [J].
Brito, Erika H. S. ;
Fontenelle, Raquel O. S. ;
Brilhante, Raimunda S. N. ;
Cordeiro, Rossana A. ;
Monteiro, Andre J. ;
Sidrim, Jose J. C. ;
Rocha, Marcos F. G. .
VETERINARY JOURNAL, 2009, 182 (02) :320-326
[6]   Virulence attributes and genetic variability of oral Candida albicans and Candida tropicalis isolates [J].
Carim da Costa, Karen Regina ;
Ferreira, Joseane Cristina ;
Sicchiroli Lavrador, Marco Aurelio ;
Baruffi, Marcelo Dias ;
Candido, Regina Celia .
MYCOSES, 2012, 55 (03) :e97-e105
[7]   Candida albicans and Candida tropicalis in Oral Candidosis: Quantitative Analysis, Exoenzyme Activity, and Antifungal Drug Sensitivity [J].
Carim da Costa, Karen Regina ;
Ferreira, Joseane Cristina ;
Komesu, Marilena Chinali ;
Candido, Regina Celia .
MYCOPATHOLOGIA, 2009, 167 (02) :73-79
[8]   Candida tropicalis in human disease [J].
Chai, Louis Yi Ann ;
Denning, David W. ;
Warn, Peter .
CRITICAL REVIEWS IN MICROBIOLOGY, 2010, 36 (04) :282-298
[9]   Activities of Triazole-Echinocandin Combinations against Candida Species in Biofilms and as Planktonic Cells [J].
Chatzimoschou, Athanasios ;
Katragkou, Spasia ;
Simitsopoulou, Maria ;
Antachopoulos, Charalampos ;
Georgiadou, Elpiniki ;
Walsh, Thomas J. ;
Roilides, Emmanuel .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2011, 55 (05) :1968-1974
[10]  
Clinical and Laboratory Standards Institute, 2008, M27A3 NCCLS