Elicitation of tropane alkaloid biosynthesis in transformed root cultures of Datura stramonium

被引：68
作者：
Zabetakis, I
Edwards, R
O'Hagan, D
机构：
[1] Univ Durham, Dept Chem, Durham DH1 3LE, England
[2] Univ Durham, Dept Biol Sci, Durham DH1 3LE, England
来源：
PHYTOCHEMISTRY | 1999年 / 50卷 / 01期
关键词：
Datura stramonium; Solanaceae; hyoscyamine; root cultures; elicitation; methyl jasmonate;
D O I：
10.1016/S0031-9422(98)00490-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Hairy root cultures of Datura stramonium were treated with methyl jasmonate (MeJa), a cell wall preparation from baker's yeast and oligogalacturonides, respectively, and analysed for the accumulation of the tropane alkaloids, littorine, hyoscyamine and scopolamine, and their precursors, phenyllactate and tropine. The treatments increased alkaloid accumulation in the order MeJa > fungal elicitor > oligogalacturonide and, in all cases, this was associated with an increase in tropine but a decline in phenyllactate concentrations. Time-course studies following MeJa treatments confirmed that increased tropane alkaloid synthesis was due to the differential enhancement of tropine biosynthesis. (C) 1998 Published by Elsevier Science Ltd. All rights reserved.
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页码：53 / 56
页数：4
相关论文
共 17 条
[1]   Signalling molecules and the synthesis of alkaloids in Catharanthus roseus seedlings [J].
Aerts, RJ ;
Schafer, A ;
Hesse, M ;
Baumann, TW ;
Slusarenko, A .
PHYTOCHEMISTRY, 1996, 42 (02) :417-422
[2]   METHYL JASMONATE VAPOR INCREASES THE DEVELOPMENTALLY CONTROLLED SYNTHESIS OF ALKALOIDS IN CATHARANTHUS AND CINCHONA SEEDLINGS [J].
AERTS, RJ ;
GISI, D ;
DECAROLIS, E ;
DELUCA, V ;
BAUMANN, TW .
PLANT JOURNAL, 1994, 5 (05) :635-643
[3]   Structural separation of biological activities of jasmonates and related compounds [J].
Blechert, S ;
Bockelmann, C ;
Brümmer, O ;
Füsslein, M ;
Gundlach, H ;
Haider, G ;
Hölder, S ;
Kutchan, TM ;
Weiler, EW ;
Zenk, MH .
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1997, (23) :3549-3559
[4]   ABSCISSION-INDUCING PROPERTIES OF METHYL JASMONATE, ABA, AND ABA-METHYL ESTER AND THEIR INTERACTIONS WITH ETHEPHON, AGNO3, AND MALFORMIN [J].
CURTIS, RW .
JOURNAL OF PLANT GROWTH REGULATION, 1984, 3 (03) :157-168
[5]   SIGNAL MOLECULES IN SYSTEMIC PLANT-RESISTANCE TO PATHOGENS AND PESTS [J].
ENYEDI, AJ ;
YALPANI, N ;
SILVERMAN, P ;
RASKIN, I .
CELL, 1992, 70 (06) :879-886
[6]   JASMONIC ACID IS A SIGNAL TRANSDUCER IN ELICITOR-INDUCED PLANT-CELL CULTURES [J].
GUNDLACH, H ;
MULLER, MJ ;
KUTCHAN, TM ;
ZENK, MH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (06) :2389-2393
[7]  
Hahn MG, 1992, MOL PLANT PATHOL, V2, P103
[8]   ALKALOID BIOGENESIS - MOLECULAR ASPECTS [J].
HASHIMOTO, T ;
YAMADA, Y .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1994, 45 :257-285
[9]   12-OXO-PHYTODIENOIC ACID INDUCES ACCUMULATION OF BERBERINE BRIDGE ENZYME TRANSCRIPT IN A MANNER ANALOGOUS TO METHYL JASMONATE [J].
KUTCHAN, TM .
JOURNAL OF PLANT PHYSIOLOGY, 1993, 142 (04) :502-505
[10]   Tropic acid ester biosynthesis in Datura stramonium and related species [J].
O'Hagan, D ;
Robins, RJ .
CHEMICAL SOCIETY REVIEWS, 1998, 27 (03) :207-212
12