Antibody Response of Buffalo Calves to Different Levels of Foot and Mouth Disease Virus Immunogen

Antibody response of buffalo calves to different levels of Foot and Mouth Disease (FMD) virus immunogen was investigated. Vaccine containing 106.2 units of immunogen/TCID50 of FMD virus (O, A and Asia-1) serotypes induced log(2) (1.3 +/- 0.4) units of anti-FMD O Complement Fixing Geometric Mean antibody (FMD O CFT-CGM) titer, log(2) (1.4 +/- 0.3) units of anti-FMD A CFT-CGM titer and log(2) (2.0 +/- 0.7) units of anti-FMD Asia-1 CFT-CGM titer. The vaccine containing 2x106.2 units of immunogen of each of the virus serotypes induced log(2) (2.2 +/- 0.2) units of anti-FMD O CFT-CGM titer, log(2) (2.1 +/- 0.25) units of anti-FMD A CFT-CGM titer and log(2) (3.4 +/- 0.8) units of anti-FMD Asia-1 CFT-CGM titer. The vaccine containing 3x10(6.2) units of TCID50 of each of the virus serotypes induced log(2) (5.3 +/- 2.0) units of anti-FMD O CFT-CGM titer, log(2) (4.6 +/- 1.9) units of anti-FMD A CFT-CGM titer and log(2) (5.0 +/- 2.2) units of anti-FMD Asia-1 CFT-CGM titer. Moreover, buffalo calves (n=3) which were primed and boosted with 60 days interval using vaccine containing 2x10(6.2) units of immunogen of each of the virus serotype, showed log(2)5.0 and log(2)6.3 units of anti FMD O CFT-GMT antibody titer, log(2)4.6 and log(2)6.0 units of anti FMD A CFT GMT antibody titer, log(2)5.6 and log(2)6.0 units of anti FMD Asia-1 CFT GMT antibody titer, on 30 and 120 days post boosting. Antibody response of buffalo calves was directly proportional to amount of FMD virus immunogen serotypes in the vaccine.