Comparison of structures and anticoagulant activities of fucosylated chondroitin sulfates from different sea cucumbers

被引：229
作者：
Chen, Shiguo [1 ]
Xue, Changhu [1 ]
Yin, Li'ang [1 ]
Tang, Qingjuan [1 ]
Yu, Guangli [1 ]
Chai, Wengang [2 ,3 ]
机构：
[1] Ocean Univ China, Coll Food Sci & Technol, Qingdao 266003, Peoples R China
[2] Ocean Univ China, Sch Med & Pharm, Qingdao 266003, Peoples R China
[3] Imperial Coll London, Glycosci Lab, Fac Med, Harrow HA1 3UJ, Middx, England
来源：
CARBOHYDRATE POLYMERS | 2011年 / 83卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Sea cucumber; Fucosylated chondroitin sulfate; Chondroitin sulfate; NMR; Anticoagulant activity; DEPOLYMERIZED HOLOTHURIAN GLYCOSAMINOGLYCAN; HEPARIN-COFACTOR-II; STICHOPUS-JAPONICUS; TRITERPENE GLYCOSIDES; FUCOSE BRANCHES; BODY-WALL; POLYSACCHARIDE ACCOUNT; FUCOIDAN FRACTION; ECHINODERM; ACTIVATION;
D O I：
10.1016/j.carbpol.2010.08.040
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Fucosylated chondroitin sulfates (fCSs) were isolated from four sea cucumbers Pearsonothuria graeffet (Indo-Pacific) Stichopus tremulus (Western Indian Ocean) Holothuria vagabunda (Norwegian coast) and Isostichopus badionotus (Western Atlantic) The detailed sequences of fCSs particularly their fucose branches were characterized and compared H-1 and C-13 NMR of the polysaccharides clearly identified three different sulfation patterns on the branched fucoses 4-0-mono- 2 3-0-di- and 2 4-0-di-sulfation variously distributed in the four fCSs The chondroitin sulfate backbones were established based on the monosaccharide composition of the polysaccharides and two-dimensional NMR of their oligosaccharide fragments Anticoagulant activities of the four fCSs were assessed and compared The results indicated that the difference in their anticoagulant activities can be attributed to the difference in sulfation pattern of the fucose branch of the chondroitin sulfate and 2 4-0-disulfation is important for anticoagulant activity (C) 2010 Elsevier Ltd All rights reserved
引用
收藏
页码：688 / 696
页数：9
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