Inhibitory Effect of Extracellular Polysaccharide EPS-II from Pseudoalteromonas on Candida adhesion to Cornea in vitro

被引：4

作者：

Chen Hao ^{[1
]}

Zheng Zhou ^{[2
]}

Chen Peng ^{[1
]}

Wu Xiang Gen ^{[1
]}

Zhao Ge ^{[1
]}

机构：

[1] Shandong Eye Inst, Shandong Prov Key Lab Ophthalmol, State Key Lab Cultivat Base, Qingdao 266071, Shandong, Peoples R China

[2] State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Shandong, Peoples R China

来源：

BIOMEDICAL AND ENVIRONMENTAL SCIENCES | 2012年 / 25卷 / 02期

关键词：

Extracellular polysaccharide EPS-II; Candida albicans; Cornea; Adhesion; Inhibitory effect; FUNGAL KERATITIS; PATHOGENIC FUNGI; ADHERENCE; CELLS; DERIVATIVES; EXPRESSION;

D O I：

10.3967/0895-3988.2012.02.013

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Objective Fungal keratitis (FK) is a vision-threatening infection, whose treatment requires more effective and safer anti-fungal agent exploitation urgently. With this aim, we focused on the effect of an extracellular polysaccharide on fungal adhesion to human corneal epithelial cells. Methods We performed the cytotoxicity assays of the extracellular polysaccharide EPS-II from an antarctic bacterium Pseudoaltermonas and evaluated its inhibitory effect on Candida albicans cells' adherence to human corneal epithelial cells (HCECs). Results EPS-II, which displayed minor cytotoxicity but also promoted proliferation of HCECs, could inhibit the adherence of yeast cells to HCECs in a dose-dependent manner. EPS-II could also suppress the subsequent PI3K/AKT signaling pathway, and thereby decrease the expression of early inflammatory cytokines. Conclusions Extracellular polysaccharide EPS-II was suggested as a new natural agent for attenuating FK.

引用

页码：210 / 215

页数：6

共 26 条

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