Stabilised Biosensing Using Needle-Based Recess Electrodes

被引：8

作者：

Anastasova, Salzitsa ^{[1
]}

Spehar-Deleze, Anna-Maria ^{[1
]}

Bickham, Dale ^{[1
]}

Uebel, Patrick ^{[2
]}

Schmidt, Markus ^{[2
]}

Russell, Philip ^{[2
]}

Vadgama, Pankaj ^{[1
]}

机构：

[1] Univ London, London, England

[2] Max Planck Inst Sci Light, D-91058 Erlangen, Germany

来源：

ELECTROANALYSIS | 2012年 / 24卷 / 03期

基金：

英国工程与自然科学研究理事会;

关键词：

Recessed tip electrode; Oxygen; Glucose; Biofouling; Selectivity; in vivo; IN-VIVO; GLUCOSE BIOSENSORS; EXTENDED LINEARITY; ENZYME ELECTRODE; OXYGEN DELIVERY; SENSOR; DIFFUSION; MEMBRANE; PERFORMANCE; TRANSPORT;

D O I：

10.1002/elan.201100511

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A recess disk electrode for amperometric monitoring of oxygen and glucose is reported. The basic design of sensor is a needle structure for easy implantation, embodying a gold filled silica capillary with a 80 mu m inner diameter working electrode. Recess and inlaid disc electrodes, placed inside stainless steel tubes which served as a counter/reference electrode, were compared. The working electrode surface was modified with different treatments and barrier membranes to achieve selectivity for the analytes of interest. The basic needle format is ideal for in vivo use, and the format is easily extendable to other analyte targets. The sensors show linear working range for oxygen up to 160 mmHg partial pressure of oxygen, and for glucose from 1 to 10 mM. Bio-fouling, as assessed by exposure to bovine serum albumin, was significantly reduced. Response times for the recess construct was increased but remained within the acceptable range for physiological monitoring. The operational stability of the sensors is demonstrated as well as the interference-free detection of peroxide in the presence of physiologically relevant levels of ascorbic acid, uric acid, acetaminophen, and catechol. Preliminary in vivo tests showed excellent response towards glucose.

引用

页码：529 / 538

页数：10

共 41 条

[1] The determination of diffusion coefficients of counter ion in an ion exchange membrane using electrical conductivity measurement [J].