X-Ray Reflectivity and Diffraction Studies of Doxorubicin Binding to Model Lipid Membranes

被引：2

作者：

Novikova, Natalia ^{[1
]}

Kovalchuk, Mikhail ^{[1
]}

Konovalov, Oleg ^{[2
]}

Stepina, Nina ^{[3
]}

Rogachev, Alexandr ^{[1
]}

Belov, Alexey ^{[4
,5
]}

Maksimenko, Olga ^{[5
]}

Tarasov, Vadim ^{[4
]}

Gelperina, Svetlana ^{[5
]}

Kreuter, Joerg ^{[4
,6
]}

Yakunin, Sergey ^{[1
]}

机构：

[1] Kurchatov Inst, Natl Res Ctr, Akad Kurchatova Pl 1, Moscow 123182, Russia

[2] European Synchrotron Radiat Facil, 71 Ave Martyrs, F-38043 Grenoble 9, CS, France

[3] Russian Acad Sci, Fed Sci Res Ctr Crystallog & Photon, Shubnikov Inst Crystallog, Leninsky Pr 59, Moscow 117333, Russia

[4] IM Sechcnov First Moscow State Med Univ, Sechenov Univ, Tnibetskaya St 8-2, Moscow 119991, Russia

[5] D Mendeleev Univ Chem Technol, Miusskaya Pl 9, Moscow 125047, Russia

[6] Goethe Univ, Inst Pharmaceut Technol, Bioctr Niederursel, Max von Laue Str 9, D-60438 Frankfurt, Germany

来源：

BIONANOSCIENCE | 2020年 / 10卷 / 03期

关键词：

Doxorubicin; Langmuir monolayer; X-ray reflectivity; GID;

D O I：

10.1007/s12668-020-00742-0

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

The effect of anticancer antibiotic doxorubicin on structural organization of anionic lipid monolayers has been studied. X-ray reflectivity and grazing incidence diffraction techniques were applied to monitor the changes in 2D structure and electron density distribution of Langmuir monolayer composed of negatively charged dipalmitoylphosphatidylglycerol (DPPG) and dioleoylphosphatidylserine (DOPS). For comparison, monolayer of zwitterionic dipalmitoylphosphatidylethanolamine (DPPE) also was investigated. The presented experimental results suggest that doxorubicin interaction with anionic lipid monolayers (DPPG and DOPS) proceeds preferentially via electrostatic attraction-positively charged amino groups of doxorubicin bind to negatively charged head groups of phospholipid molecules. Based on the obtained data, the penetration of doxorubicin into the hydrophobic part of anionic lipid monolayers does not occur. X-ray measurements on DPPE monolayer indicated that doxorubicin did not cause any significant alterations of molecular packing in condensed monolayer of zwitterionic DPPE molecules.

引用

页码：618 / 624

页数：7

共 15 条

[1] Daunorubicin and doxorubicin molecular interplay with 2D membrane models [J].