Detection of viral DNA sequences in sporadic colorectal cancers in relation to CpG island methylation and methylator phenotype

被引:48
作者
Karpinski, Pawel [1 ]
Myszka, Aleksander [2 ]
Ramsey, David [3 ]
Kielan, Wojciech [4 ]
Sasiadek, Maria Malgorzata [1 ]
机构
[1] Wroclaw Med Univ, Dept Genet, PL-50368 Wroclaw, Poland
[2] Univ Rzeszow, Dept Genet, Rzeszow, Poland
[3] Univ Limerick, Dept Math & Stat, Limerick, Ireland
[4] Wroclaw Med Univ, Dept Gen & Oncol Surg 2, PL-50368 Wroclaw, Poland
关键词
CIMP; Virus; JCV; EBV; CRC; Colorectal; EPSTEIN-BARR-VIRUS; HUMAN-PAPILLOMAVIRUS DNA; JC VIRUS; CHROMOSOMAL INSTABILITY; BRAF MUTATION; T-ANTIGEN; ASSOCIATION; CARCINOMAS; EXPRESSION; INFECTION;
D O I
10.1007/s13277-011-0165-6
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
There is evidence that insertion of viral DNA into a mammalian genome can lead to alterations of methylation patterns. The aim of the present study was to examine the presence of DNA sequences of five human DNA viruses (assessed by PCR): JC polyoma virus (JCV), human adenovirus (AdV), Epstein-Barr virus (EBV), Kaposi sarcoma-associated herpesvirus (KSHV/HHV8) and human papillomavirus (HPV) in a cohort of 186 sporadic colorectal cancers (CRCs) and related these data with the methylation status of six CpG island methylator phenotype (CIMP)-specific genes (MLH1, CACNA1G, NEUROG1, IGF2, SOCS1, RUNX3) and seven cancer-related genes markers (p16, MINT1, MINT2, MINT31, EN1, SCTR and INHBB) assessed by methylation-specific PCR in 186 and 134 CRC cases, respectively. The AdV, KSHV and HPV were detected in four (2%), two (1%) and zero CRC cases, respectively, and thus were excluded from further analyses. Although 19% and 9% of the CRCs were positive for EBV and JCV, respectively, no associations between virus presence and CpG island methylation were found after correction for multiple testing. Our results demonstrate that the presence of DNA sequences of JCV and EBV in CRC is unrelated to the methylation of the 13 cancer-related CpG islands and CIMP.
引用
收藏
页码:653 / 659
页数:7
相关论文
共 41 条
[21]   Adenovirus small e1a alters global patterns of histone modification [J].
Horwitz, Gregory A. ;
Zhang, Kangling ;
McBrian, Matthew A. ;
Grunstein, Michael ;
Kurdistani, Siavash K. ;
Berk, Arnold J. .
SCIENCE, 2008, 321 (5892) :1084-1085
[22]   Opinion - CpG island methylator phenotype in cancer [J].
Issa, JP .
NATURE REVIEWS CANCER, 2004, 4 (12) :988-993
[23]   The CpG island methylator phenotype correlates with long-range epigenetic silencing in colorectal cancer [J].
Karpinski, Pawel ;
Ramsey, David ;
Grzebieniak, Zygmunt ;
Sasiadek, Maria M. ;
Blin, Nikolaus .
MOLECULAR CANCER RESEARCH, 2008, 6 (04) :585-591
[24]   Aberrant epigenetic patterns in the etiology of gastrointestinal cancers [J].
Karpinski, Pawel ;
Sasiadek, Maria M. ;
Blin, Nikolaus .
JOURNAL OF APPLIED GENETICS, 2008, 49 (01) :1-10
[25]   Epstein-Barr virus involvement is mainly restricted to lymphoepithelial type of gastric carcinoma among various epithelial neoplasms [J].
Kijima, Y ;
Hokita, S ;
Takao, S ;
Baba, M ;
Natsugoe, S ;
Yoshinaka, H ;
Aridome, K ;
Otsuji, T ;
Itoh, T ;
Tokunaga, M ;
Eizuru, Y ;
Aikou, T .
JOURNAL OF MEDICAL VIROLOGY, 2001, 64 (04) :513-518
[26]   Prevalence of infectious pathogens in Crohn's disease [J].
Knoesel, Thomas ;
Schewe, Christiane ;
Petersen, Nanni ;
Dietel, Manfred ;
Petersen, Iver .
PATHOLOGY RESEARCH AND PRACTICE, 2009, 205 (04) :223-230
[27]  
Liu HX, 2003, WORLD J GASTROENTERO, V9, P2464
[28]   JC virus and human colon carcinoma: An intriguing and inconclusive association [J].
Losa, JH ;
Fernandez-Soria, V ;
Parada, C ;
Sanchez-Prieto, R ;
Cajal, SRY ;
Fedele, CG ;
Tenorio, A .
GASTROENTEROLOGY, 2003, 124 (01) :268-269
[29]  
Müller K, 2001, J BIOL CHEM, V276, P14271
[30]   JC Virus T-Antigen in Colorectal Cancer Is Associated with p53 Expression and Chromosomal Instability, Independent of CpG Island Methylator Phenotype [J].
Nosho, Katsuhiko ;
Shima, Kaori ;
Kure, Shoko ;
Irahara, Natsumi ;
Baba, Yoshifumi ;
Chen, Li ;
Kirkner, Gregory J. ;
Fuchs, Charles S. ;
Ogino, Shuji .
NEOPLASIA, 2009, 11 (01) :87-95