A Turn-On Sensor for Highly Sensitive and Selective Detect Heparin in Human Serum Albumin

被引：2

作者：

Huang, Cuiping ^{[1
]}

Cao, Jian ^{[1
]}

Liu, Mingming ^{[1
]}

Tu, Yajing ^{[1
]}

Zhang, Meijuan ^{[1
]}

Zheng, Junying ^{[1
]}

机构：

[1] Shanghai Univ Engn Sci, Sch Chem & Chem Engn, 333 Longteng Rd, Shanghai 201620, Peoples R China

来源：

CHEMISTRYSELECT | 2022年 / 7卷 / 15期

关键词：

fluorescence; heparin; PRTM; sensor; turn-on; MASS-SPECTROMETRY; SULFATE; BINDING;

D O I：

10.1002/slct.202200363

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel positively charged sensor, NAP-MBT+, was designed and constructed by introducing a 2-methylbenzothiazole cation (MBT+) into naphthalimide derivative (NAP-phCHO) via a double carbon bond. NAP-MBT+ can realize the detection sensitively and selectively for heparin (Hep) by "turn-on" fluorescence emission in several seconds. NAP-MBT+ displayed the very significant fluorescence emission enhancement only to Hep among various interference from counter ions and biomolecules. The fluorescence emission of NAP-MBT+ to Hep depended on the electrostatic attraction between the positive and negative charges. NAP-MBT+-Hep assembly can be dissociated by protamine (PRTM) appearing an "off-on-off" signal because of the stronger affinity of Hep to PRTM. NAP-MBT+ was successfully applied to detect Hep in 0.2 % human serum.

引用

页数：8

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