Cloning of full-length genomic DNA encoding human FcεRI α-chain and its transcriptional regulation

被引：12

作者：

Nishiyama, C

Hasegawa, M

Nishiyama, M

Takahashi, K

Yokota, T

Okumura, K

Ra, C

机构：

[1] Juntendo Univ, Sch Med, Allergy Res Ctr, Bunkyo Ku, Tokyo 1138421, Japan

[2] Juntendo Univ, Sch Med, Dept Immunol, Bunkyo Ku, Tokyo 1138421, Japan

[3] Asahi Breweries Ltd, Foods & Pharmaceut Res & Dev Lab, Ibaraki, Osaka 3020106, Japan

[4] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo 1138647, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 284卷 / 04期

关键词：

Fc epsilon RI alpha-chain; promoter; genomic DNA;

D O I：

10.1006/bbrc.2001.5079

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two novel exons, named exon 1A and exon 2A, were found at 18.4 and 12.6 kb upstream from the exon known as the first exon of human Fc is an element of RI alpha -chain gene. Transcription from the promoter present in the upstream of exon 1A was decreased by mutations introduced into the "first intron" between exon IA and exon 2A, suggesting the presence of an intronic regulatory element in the intron. Consistent with this, electrophoretic mobility shift assay revealed the presence of a nuclear factor which bound the region in Fc is an element of RI alpha -chain positive cells. (C) 2001 Academic Press.

引用

页码：1056 / 1064

页数：9

共 29 条

[21] Nasal mast cells in perennial allergic rhinitics exhibit increased expression of the Fc epsilon RI, CD40L, IL-4, and IL-13, and can induce IgE synthesis in B cells [J].

Pawankar, R ;

Okuda, M ;

Yssel, H ;

Okumura, K ;

Ra, CS .

JOURNAL OF CLINICAL INVESTIGATION, 1997, 99 (07) :1492-1499

[22]

PFFERKORN LC, 1994, J IMMUNOL, V153, P3228

[23]

RA C, 1989, J BIOL CHEM, V264, P15323

[24] HUMAN AND RAT MAST-CELL HIGH-AFFINITY IMMUNOGLOBULIN-E RECEPTORS - CHARACTERIZATION OF PUTATIVE ALPHA-CHAIN GENE-PRODUCTS [J].

SHIMIZU, A ;

TEPLER, I ;

BENFEY, PN ;

BERENSTEIN, EH ;

SIRAGANIAN, RP ;

LEDER, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (06) :1907-1911

[25]

Takahashi K, 2001, EUR J IMMUNOL, V31, P590, DOI 10.1002/1521-4141(200102)31:2<590::AID-IMMU590>3.3.CO

[26]

2-4

[27] IL-4 upregulates Fc epsilon RI alpha-chain messenger RNA in eosinophils [J].

Terada, N ;

Konno, A ;

Terada, Y ;

Fukuda, S ;

Yamashita, T ;

Abe, T ;

Shimada, H ;

Ishida, K ;

Yoshimura, K ;

Tanaka, Y ;

Ra, C ;

Ishikawa, K ;

Togawa, K .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1995, 96 (06) :1161-1169

[28]

Toru H, 1996, INT IMMUNOL, V8, P1367

[29]

Xia HZ, 1997, J IMMUNOL, V159, P2911

← 1 2 3 →