Insecticidal proteins from Bacillus thuringiensis protect corn from corn rootworms

被引：153
作者：
Moellenbeck, DJ
Peters, ML
Bing, JW
Rouse, JR
Higgins, LS
Sims, L
Nevshemal, T
Marshall, L
Ellis, RT
Bystrak, PG
Lang, BA
Stewart, JL
Kouba, K
Sondag, V
Gustafson, V
Nour, K
Xu, DP
Swenson, J
Zhang, J
Czapla, T
Schwab, G
Jayne, S
Stockhoff, BA
Narva, K
Schnepf, HE
Stelman, SJ
Poutre, C
Koziel, M
Duck, N
机构：
[1] Pioneer HiBred Int Inc, Johnston, IA 50131 USA
[2] Dow AgroSci LLC, Huxley, IA 50124 USA
[3] Dow AgroSci LLC, San Diego, CA 92121 USA
来源：
NATURE BIOTECHNOLOGY | 2001年 / 19卷 / 07期
关键词：
D O I：
10.1038/90282
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Field tests of corn co-expressing two new delta-endotoxins from Bacillus thuringiensis (Bt) have demonstrated protection from root damage by western corn rootworm (Diabrotica virgifera virgifera LeConte). The level of protection exceeds that provided by chemical insecticides. In the bacterium, these proteins form crystals during the sporulation phase of the growth cycle, are encoded by a single operon, and have molecular masses of 14 kDa and 44 kDa. Corn rootworm larvae fed on corn roots expressing the proteins showed histopathological symptoms in the midgut epithelium.
引用
收藏
页码：668 / 672
页数：5
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