Microfluidic high-throughput single-cell mechanotyping: Devices and applications

被引：11

作者：

Choi, Gihoon ^{[1
]}

Tang, Zifan ^{[1
]}

Guan, Weihua ^{[1
,2
,3
]}

机构：

[1] Penn State Univ, Dept Elect Engn, University Pk, PA 16802 USA

[2] Penn State Univ, Dept Biomed Engn, University Pk, PA 16802 USA

[3] Sandia Natl Labs, Dept Biotechnol & Bioengn, Livermore, CA 94550 USA

来源：

NANOTECHNOLOGY AND PRECISION ENGINEERING | 2021年 / 4卷 / 04期

基金：

美国国家科学基金会;

关键词：

Microfluidic; Single-cell; Cell deformability; Mechanotyping; RED-BLOOD-CELLS; SUSPENDED MICROCHANNEL RESONATORS; DEFORMABILITY-BASED SEPARATION; CIRCULATING TUMOR-CELLS; MECHANICAL-PROPERTIES; OPTICAL DEFORMABILITY; PARTICLE SEPARATION; CANCER-CELLS; ELASTICITY; FILTRATION;

D O I：

10.1063/10.0006042

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The mechanical behavior of individual cells plays an important role in regulating various biological activities at the molecular and cellular levels. It can serve as a promising label-free marker of cells' physiological states. In the past two decades, several techniques have been developed for understanding correlations between cellular mechanical changes and human diseases. However, numerous technical challenges remain with regard to realizing high-throughput, robust, and easy-to-perform measurements of single-cell mechanical properties. In this paper, we review the emerging tools for single-cell mechanical characterization that are provided by microfluidic technology. Different techniques are benchmarked by considering their advantages and limitations. Finally, the potential applications of microfluidic techniques based on cellular mechanical properties are discussed. (C) 2021 Author(s).

引用

页数：18