Resistive-Pulse Measurements with Nanopipettes: Detection of Vascular Endothelial Growth Factor C (VEGF-C) Using Antibody-Decorated Nanoparticles

被引：38

作者：

Cai, Huijing ^{[1
]}

Wang, Yixian ^{[1
]}

Yu, Yun ^{[1
]}

Mirkin, Michael V. ^{[1
]}

Bhakta, Snehasis ^{[2
]}

Bishop, Gregory W. ^{[2
]}

Joshi, Amit A. ^{[2
]}

Rusling, James F. ^{[2
,3
,4
]}

机构：

[1] CUNY Queens Coll, Dept Chem & Biochem, Flushing, NY 11367 USA

[2] Univ Connecticut, Dept Chem, Storrs, CT 06269 USA

[3] Univ Connecticut, Ctr Hlth, Dept Cell Biol, Farmington, CT USA

[4] Natl Univ Ireland Galway, Sch Chem, Galway, Ireland

来源：

ANALYTICAL CHEMISTRY | 2015年 / 87卷 / 12期

基金：

美国国家科学基金会;

关键词：

ION-TRANSFER; MICROSCOPY; CANCER; LYMPHANGIOGENESIS; MOLECULES; BIOMARKER; NANOPORES; PROBES;

D O I：

10.1021/acs.analchem.5b01468

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Quartz nanopipettes have recently been employed for resistive-pulse sensing of Au nanoparticles (AuNP) and nanoparticles with bound antibodies. The analytical signal in such experiments is the change in ionic current caused by the nanoparticle translocation through the pipette orifice. This paper describes resistive-pulse detection of cancer biomarker (Vascular Endothelial Growth Factor-C, VEGF-C) through the use of antibody-modified AuNPs and nanopipettes. The main challenge was to differentiate between AuNPs with attached antibodies for VEGF-C and antigen-conjugated particles. The zeta-potentials of these types of particles are not very different, and, therefore, carefully chosen pipettes with well-characterized geometry were necessary for selective detection of VEGF-C.

引用

页码：6403 / 6410

页数：8

共 50 条

[1] Compartmental Genomics in Living Cells Revealed by Single-Cell Nanobiopsy [J].

Actis, Paolo ;

Maalouf, Michelle M. ;

Kim, Hyunsung John ;

Lohith, Akshar ;

Vilozny, Boaz ;

Seger, R. Adam ;

Pourmand, Nader .

ACS NANO, 2014, 8 (01) :546-553

[2]

Actis Paolo, 2010, Bioanal Rev, V1, P177

[3]

Amemiya S, 2014, ELECTROCHEM-SP PER R, V12, P1, DOI 10.1039/9781849737333-00001

[4] Resistive-pulse sensing - From microbes to molecules [J].

Bayley, H ;

Martin, CR .

CHEMICAL REVIEWS, 2000, 100 (07) :2575-2594

[5] The potential and challenges of nanopore sequencing [J].

Branton, Daniel ;

Deamer, David W. ;

Marziali, Andre ;

Bayley, Hagan ;

Benner, Steven A. ;

Butler, Thomas ;

Di Ventra, Massimiliano ;

Garaj, Slaven ;

Hibbs, Andrew ;

Huang, Xiaohua ;

Jovanovich, Stevan B. ;

Krstic, Predrag S. ;

Lindsay, Stuart ;

Ling, Xinsheng Sean ;

Mastrangelo, Carlos H. ;

Meller, Amit ;

Oliver, John S. ;

Pershin, Yuriy V. ;

Ramsey, J. Michael ;

Riehn, Robert ;

Soni, Gautam V. ;

Tabard-Cossa, Vincent ;

Wanunu, Meni ;

Wiggin, Matthew ;

Schloss, Jeffery A. .

NATURE BIOTECHNOLOGY, 2008, 26 (10) :1146-1153

[6] Nanopipette delivery of individual molecules to cellular compartments for single-molecule fluorescence tracking [J].

Bruckbauer, Andreas ;

James, Peter ;

Zhou, Dejian ;

Yoon, Ji Won ;

Excell, David ;

Korchev, Yuri ;

Jones, Roy ;

Klenerman, David .

BIOPHYSICAL JOURNAL, 2007, 93 (09) :3120-3131

[7] Probing rapid ion transfer across a nanoscopic liquid-liquid interface [J].

Cai, CX ;

Tong, YH ;

Mirkin, MV .

JOURNAL OF PHYSICAL CHEMISTRY B, 2004, 108 (46) :17872-17878

[8] Scanning Ion Conductance Microscopy [J].

Chen, Chiao-Chen ;

Zhou, Yi ;

Baker, Lane A. .

ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 5, 2012, 5 :207-228

[9] Integrated Ultramicroelectrode-Nanopipet Probe for Concurrent Scanning Electrochemical Microscopy and Scanning Ion Conductance Microscopy [J].

Comstock, David J. ;

Elam, Jeffrey W. ;

Pellin, Michael J. ;

Hersam, Mark C. .

ANALYTICAL CHEMISTRY, 2010, 82 (04) :1270-1276

[10] Method of Creating a Nanopore-Terminated Probe for Single-Molecule Enantiomer Discrimination [J].

Gao, Changlu ;

Ding, Shu ;

Tan, Qiulin ;

Gu, Li-Qun .

ANALYTICAL CHEMISTRY, 2009, 81 (01) :80-86

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